Address: 45-1 Ramsey Road, Shirley, NY 11967, USA    USA: 1-631-559-9269  1-631-448-7888   Fax: 1-631-938-8127  Europe: 44-207-048-3343   Email: info@creative-biomart.com
Home > Products > Assay Kits > Cellular Kits > Cell Viability NIR Fluorescence Assay Kit

Cell Viability NIR Fluorescence Assay Kit

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His(1)
Cat.No.:
Kit-0202
Product Overview:
Cell Viability Assay Kit (NIR Fluorescence) is a fluorescent assay used to label cells for the research of cellular functions.
Description:
The far red fluorescent dye used in the kit is a hydrophobic compound that can easily permeate intact live cells and gets enhanced fluorescence upon entering into live cells. The hydrolysis of the weakly fluorescent substrate by intracellular esterases generates a strongly red fluorescent hydrophilic product that is well-retained in the cell cytoplasm. The red fluorophore generated by the weakly fluorescent substrate has the spectral properties similar to those of Cy5 or Alexa Fluor 647 at Ex/Em = ~640/660 nm (Cy5 or Alexa Fluor 647 are the trademarks of GE Healthcare and Invitrogen respectively). When well excited with the He-Ne Laser at 633 nm, the red fluorophore emits intense red fluorescence at ~660 nm.
Applications:
The Cell Viability Assay Kit (NIR Fluorescence) is a set of tool for monitoring cell viability and cellular functions.
Usage:
For research use only (RUO)
Storage:
Keep in freezer and protect from light.
Kit Components:
Component A: CytoCalcein NIR (lyophilized) 2 vialsComponent B: DMSO 100 μLComponent C: Assay Buffer 10 mL x 2
Detection method:
Fluorometric
Compatible Sample Types:
Cells
Features & Benefits:
Robust: Higher maximum signal with lower variation across the plate.Convenient: Formulated to have minimal hands-on time.Rapid Dye Loading: Dye loading at RT for 30 min to 1 hr.Versatile applications: Compatible with many cell lines and targets.


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Optional requirements on this protein +Expand
C-fusion   N-fusion  Non-tagged
His   GST  Fc  Others
<1.0 eu/μg   <0.1 eu/μg  <0.01 eu/μg  Not required
Monomer Isolation   Dimer Isolation   Not required
>80% by SDS-PAGE   >90% by SDS-PAGE  >95% by SDS-PAGE  Others
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45-1 Ramsey Road, Shirley, NY 11967, USA
USA: 1-631-559-9269  1-631-448-7888
Europe: 44-207-048-3343
FAX: 1-631-938-8127
EMAIL: info@creative-biomart.com

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