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Home > Products > Assay Kits > Signaling Kits > Cellular Calcineurin Phosphatase Activity Colorimetric Assay Kit

Cellular Calcineurin Phosphatase Activity Colorimetric Assay Kit

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Cat.No.:
Kit-0150
Product Overview:
Cellular Calcineurin Phosphatase Activity Assay Kit (Colorimetric) is a complete colorimetric assay kit designed for measuring cellular calcineurin (CaN, PP2B) phosphatase activity. It employs a convenient 96-well microtiter-plate format with all reagents necessary for measuring calcineurin (PP2B) phosphatase activity in tissue/cellular extracts. The detection of free-phosphate released is based on the classic Malachite green assay and offers the following advantages: Non-radioactive; convenient 1-step detection; excellent sensitivity. This kit includes human recombinant calcineurin as a positive control. The RII phosphopeptide substrate (Calcineurin Substrate), supplied with this kit, is the most efficient and outstanding peptide substrate known for calcineurin.
Size:
1 x 96 tests
Applications:
Functional Studies more details
Kit Components:
Components: 1 x 96 tests; 2X EGTA Buffer: 1 x 1ml; 96-well Clear Microplate (1/2 Volume): 2 units; Active Calcineurin Enzyme: 1 x 10µl; Calcineurin Assay Buffer: 1 x 20ml; Calcineurin Substrate: 1 x 1.5mg; Calmodulin: 1 x 100µl; Desalting Column: 1 unit; Desalting Resin: 1 x 1g; Green Assay Reagent: 1 x 20ml; Lysis Buffer: 1 x 40ml; Okadaic Acid: 1 x 325µl; Phosphate Standard: 1 x 0.5ml; Protease Inhibitor Cocktail: 2 units
Detection method:
Colorimetric
Compatible Sample Types:
Cell culture extracts, Tissue Extracts


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Optional requirements on this protein +Expand
C-fusion   N-fusion  Non-tagged
His   GST  Fc  Others
<1.0 eu/μg   <0.1 eu/μg  <0.01 eu/μg  Not required
Monomer Isolation   Dimer Isolation   Not required
>80% by SDS-PAGE   >90% by SDS-PAGE  >95% by SDS-PAGE  Others
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45-1 Ramsey Road, Shirley, NY 11967, USA
USA: 1-631-559-9269  1-631-448-7888
Europe: 44-207-048-3343
FAX: 1-631-938-8127
EMAIL: info@creative-biomart.com

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