Address: 45-1 Ramsey Road, Shirley, NY 11967, USA    USA: 1-631-559-9269  1-631-448-7888   Fax: 1-631-938-8127  Europe: 44-207-048-3343   Email: info@creative-biomart.com
Home > Products > Assay Kits > Metabolism Kits > Coenzyme A Assay Kit

Coenzyme A Assay Kit

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"CoA" Related Products.
Cat.No.:
Kit-0233
Product Overview:
Coenzyme A Assay Kit is used for measuring the CoA level.
Description:
Coenzyme A (CoA) is composed of units derived from cysteine, pantothenic acid, and ATP. It plays important roles in the synthesis and oxidation of fatty acids, pyruvate oxidation in the citric acid cycle and many other biological processes. One of the main functions of CoA is the carrying and transfer of acyl groups. One of the most important acyl groups transferred is the acetate group, in which case the molecule is called acetyl-CoA. The acetyl group eventually finds itself incorporated into a variety of molecules such as cholesterol, acetylcholine, melatonin, heme and the TCA cycle intermediates. We have developed an easy, convenient assay to measure the CoA level in variety biological samples. In the assay, free CoA is specifically utilized to generate products which react with OxiRed Probe to generate color (λ = 570 nm) and fluorescence (Ex=535/Em=587 nm). The assay can detect 0.1 to 10 nmol of CoA (2.5-250 μM concentration range) in a variety of samples.
Target Species:
Mammals
Usage:
For research use only (RUO)
Storage:
Store kit at -20°C, protect from light. Warm CoA Assay Buffer to room temperature before use. Briefly centrifuge all small vials prior to opening.
Kit Components:
CoA Assay Buffer 25 mLOxiRed Probe (in DMSO) 0.2 mLConversion Enzyme Mix: lyophilized. 1 vialCoA Substrate 1 mLAcyl CoA Developer: lyophilized. 1 vialCoA Standard (10 μmol): lyophilized. 1 vial
Detection method:
Colorimetric, Fluorometric
Compatible Sample Types:
Biological Fluid, Cell Lysate, Culture Medium, Plasma, Serum, Tissue Lysate, Urine


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Optional requirements on this protein +Expand
C-fusion   N-fusion  Non-tagged
His   GST  Fc  Others
<1.0 eu/μg   <0.1 eu/μg  <0.01 eu/μg  Not required
Monomer Isolation   Dimer Isolation   Not required
>80% by SDS-PAGE   >90% by SDS-PAGE  >95% by SDS-PAGE  Others
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45-1 Ramsey Road, Shirley, NY 11967, USA
USA: 1-631-559-9269  1-631-448-7888
Europe: 44-207-048-3343
FAX: 1-631-938-8127
EMAIL: info@creative-biomart.com

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