Collagenase Inhibitor Screening Kit

• Cat.No.: Kit-2137
• Product Overview: Collagenase Inhibitor Screening Kit provides a quick and sensitive way for screening, studying and characterizing potential inhibitors of Collagenase. The kit uses Self-Quenched BODIPY conjugate of Gelatin (Type B) as a fluorogenic substrate to monitor the activity of Collagenase. Gelatin consists of a heterogeneous mixture of proteins of high average molecular weight derived from collagen Upon proteolytic digestion of the highly quenched BODIPY-labeled gelatin by Collagenase, the de-quenched BODIPY yields bright green fluorescence that can be assayed using a fluorometer or a fluorescence microplate reader (Ex/Em: 490 nm / 520 nm, 515 nm cutoff). In the presence of a Collagenase inhibitor, the gelatin is not digested, the dequenching of BODIPY does not occur and the fluorescent signal is not produced.

Specification

• Size: 100 assays
• Applications: Screening/studying/characterizing potential Collagenase inhibitors
• Storage: Store kit at -20°C, protected from light. Briefly centrifuge small vials prior to opening. Read the entire protocol before performing the assay.Collagenase Assay Buffer: Bring to room temperature before use. Store at -20°C or 4°C.Collagenase Substrate: Reconstitute with 210 µl Assay Buffer. Incubate the vial at 37°C for 10 min. Tap gently to mix and ensure that it is completely dissolved. Store the reconstituted substrate at -20°C.Collagenase: Aliquot and store at -20°C. Avoid repeated freeze/thaw.Inhibitor (1,10)-Phenanthroline (400 mM): Aliquot and store at -20°C. To make 100x working solution, dilute 2 µl of 1,10- Phenanthroline stock solution with 8 µl ethanol.