Address: 45-1 Ramsey Road, Shirley, NY 11967, USA    USA: 1-631-559-9269  1-631-448-7888   Fax: 1-631-938-8127  Europe: 44-207-048-3343   Email: info@creative-biomart.com
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Creatine Assay Kit

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Cat.No.:
Kit-0259
Product Overview:
Creatine Assay Kit is used for measuring creatine.
Description:
Creatine is an endogenous compound whose function is to maintain a high ATP/ADP ratio, by way of its phosphorylated form and creatine kinase. Creatine supplementation has been used in the treatment of muscular, neurological and neurodegenerative diseases, as well as a sport performance enhancer. Detection of creatine level has wide applications in research and development. The Creatine Assay Kit provides an accurate, convenient measure of creatine in a variety of biological samples. In the assay, creatine is enzymatically converted to sarcosine which is then specifically oxidized to generate a product that converts a colorless probe to an intensely red color (λmax = 570 nm), and highly fluorescent (Ex/Em = 538/587 nm) product. Creatine is therefore easily detected by either colorimetric or fluorometric methods. Detection range 0.001-10 mM Creatine.
Target Species:
Mammals
Usage:
For research use only (RUO)
Storage:
Store the kit at -20°C.
Kit Components:
Creatine Assay Buffer 25 mlCreatine Probe* (in DMSO) 0.2 mlCreatinase: lyophilized. 1 vialCreatine Enzyme mix: lyophilized. 1 vialCreatine Standard (10 μmol): lyophilized. 1 vial* Note: The fluorometric assay is ~10 times more sensitive than the colorimetric assay. Use 0.4 µl of the probe per reaction to decrease background/increase detection sensitivity significantly.
Detection method:
Colorimetric, Fluorometric
Compatible Sample Types:
Biological Fluid, Cell Culture Supernatant, Plasma, Serum, Tissue Culture Supernatant, Urine


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Optional requirements on this protein +Expand
C-fusion   N-fusion  Non-tagged
His   GST  Fc  Others
<1.0 eu/μg   <0.1 eu/μg  <0.01 eu/μg  Not required
Monomer Isolation   Dimer Isolation   Not required
>80% by SDS-PAGE   >90% by SDS-PAGE  >95% by SDS-PAGE  Others
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45-1 Ramsey Road, Shirley, NY 11967, USA
USA: 1-631-559-9269  1-631-448-7888
Europe: 44-207-048-3343
FAX: 1-631-938-8127
EMAIL: info@creative-biomart.com

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