Creatine Kinase Assay Kit is a colorimetric determination of creatine kinase activity at 340 nm.
CREATINE KINASE (CK), also known as creatine phosphokinase (CPK), is an enzyme (EC 18.104.22.168) expressed predominantly in skeletal muscle, smooth muscle and the brain. The CK enzyme consists of two subunits, which can be either B (brain type) or M (muscle type), and hence three different isoenzymes: CK-MM, CK-BB and CK-MB. CK catalyzes the conversion of creatine to phosphocreatine, consuming adenosine triphosphate (ATP) and generating adenosine diphosphate (ADP) and the reverse reaction. CK is often determined routinely in emergency patients with chest pain and acute renal failure. Elevation of CK is an indication of damage to muscle and has been associated with injury, rhabdomyolysis, myocardial infarction, myositis, myocarditis, malignant hyperthermia and neuroleptic malignant syndrome, etc. Lower levels can be an indication of alcoholic liver disease and rheumatoid arthritis.
Direct Assays: CK in serum, plasma and other biological samples.Pharmacology: effects of drugs on CK activity.
For research use only (RUO)
Store all reagents at -20°C. Shelf life: 6 months after receipt.
Assay Buffer 12 mLSubstrate Solution 1.0 mLEnzyme Mix 120 µLCalibrator 150 µL
Compatible Sample Types:
Biological Sample, Plasma, Serum
Features & Benefits:
Sensitive and accurate. Detection range: 5 to 300 U/L creatine kinase in 96-well plate assay.Convenient. The procedure involves adding a single working reagent, and reading the optical density at 20 min and 40 min at room temperature or 37°C.High-throughput. Can be readily automated as a high-throughput 96-well plate assay for thousands of samples per day