Creatine Assay Kit is a quantitative colorimetric/fluorimetric determination of creatine.
CREATINE is present in vertebrates and helps to supply energy to muscle. In humans and animals, approximately half of creatine originates from food (mainly from fresh meat). Creatine supplementation has been investigated as a possible therapeutic approach for the treatment of muscular, neuromuscular, neurological and neurodegenerative diseases.Simple, direct and automation-ready procedures for measuring creatine are popular in research and drug discovery. Creatine Assay Kit is based on enzymatic reactions leading to formation of a pink colored product.The optical density at 570 nm or fluorescence intensity at λem/ex = 590/530 nm is directly proportional to the creatine concentration in the sample.
Direct Assays: creatine in biological samples (e.g. serum, plasma, urine, saliva etc).
For research use only (RUO)
Store all components at -20°C. Shelf life: 12 months after receipt.
Assay Buffer 20 mLEnzyme A 120 μLEnzyme B 220 μLStandard: 20 mM creatine 400 μLDye Reagent 220 μL
Compatible Sample Types:
Plasma, Saliva, Serum, Urine
Features & Benefits:
High sensitivity and wide linear range. Use 10 μL sample. Linear detection range 4 to 1000 μM (colorimetric) or 0.5 to 50 μM (fluorimetric).Homogeneous and simple procedure. Simple "mix-and-measure" procedure allows reliable quantitation of creatine within 30 minutes.