Address: 45-1 Ramsey Road, Shirley, NY 11967, USA    USA: 1-631-559-9269  1-631-448-7888   Fax: 1-631-938-8127  Europe: 44-207-097-1828   Email:
Home > Products > Assay Kits > Activity Kits > Cyclooxygenase Activity Luminometric Assay Kit

Cyclooxygenase Activity Luminometric Assay Kit

"COX" Related Products.
Product Overview:
The Cyclooxygenase (COX) Activity Assay Kit (Luminometric) is a non-radioactive COX activity assay. This kit allows user to easily evaluate COX I and COX II inhibitors in a convenient 96-well plate based assay with COX Activity Assay Kit (Luminometric) uses a specific chemiluminescent substrate to detect the peroxidative activity of COX enzymes. After inhibition by NSAIDs, the direct residual activity of COX is measured by addition of a proprietary luminescent substrate and arachidonic acid. Light emission starts immediately and is directly proportional to the COX activity in the sample. The chemiluminescent signal is measured over 5 seconds.
1 x 96 tests
COX (cyclooxygenase, also known as Prostaglandin G/H synthase) is a membrane-bound enzyme responsible for the oxidation of arachidonic acid to Prostaglandin G2 (PGG2) and the subsequent reduction of PGG2 to PGH2. These reactions are the first steps in the formation of a variety of prostanoids.COX has been shown to be expressed in at least two different isoforms: a constitutively expressed form, COX1, and an inducible form, COX2. COX1 is thought to regulate a number of "housekeeping" functions such as vascular hemostasis and renal blood flow. Inflammation mediators such as growth factors, cytokines and endotoxin induce COX2 expression in a number of cellular systems.
Functional Studies more details
Kit Components:
Components: 1 x 96 tests; COX Substrate: 1 x 10ml; Ibuprofen: 1 x 2ml; Meloxicam: 1 x 2ml; Plate Sealer: 2 units; White 96 well microtiter plate: 1 unit
0.147 U/ml

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Optional requirements on this protein +Expand
C-fusion   N-fusion  Non-tagged
His   GST  Fc  Others
<1.0 eu/μg   <0.1 eu/μg  <0.01 eu/μg  Not required
Monomer Isolation   Dimer Isolation   Not required
>80% by SDS-PAGE   >90% by SDS-PAGE  >95% by SDS-PAGE  Others
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45-1 Ramsey Road, Shirley, NY 11967, USA
USA: 1-631-559-9269  1-631-448-7888
Europe: 44-207-097-1828
FAX: 1-631-938-8127

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