Cysteine Fluorometric Assay Kit

• Cat.No.: Kit-2140
• Product Overview: Cysteine Assay Kit provides a simple, sensitive, and high-throughput adaptable assay that detects physiological concentration of cysteine in a variety of biological fluids. The principle of the assay is based on the cleavage of thiol group of reduced cysteine producing a fluorophore (Ex/Em = 365/450 nm) with a stable signal, which is directly proportional to the amount of total cysteine in the sample. The assay is specific and other thiol-based amino acids do not interfere with the assay. The assay can detect as little as 10 µM of Cysteine in a variety of samples.

Specification

• Size: 100 assays
• Applications: Estimation of Cysteine in various biological samples
• Storage: Store kit at -20°C, protected from light. Briefly spin small vials prior to opening. Read entire protocol before performing the assay.CYS Assay Buffer: Store at -20°C or 4°C. Bring to room temperature (RT) before use.Enzyme Mix I: Aliquot and store at -20°C. Freeze/thaw should be limited to two times. Keep on ice during use.Enzyme Mix II: Reconstitute each vial with 1 ml of CYS Assay Buffer as needed. Store at 4°C. Keep on ice during use. Use the reconstituted Enzyme Mix II within a week.Reducing Agent: Reconstitute each vial with 220 µl of CYS Assay Buffer as needed. Store at 4°C. Keep on ice during use. Use the reconstituted Reducing Agent within a week.HCY Blocker: Bring to room temperature. Aliquot and store at -20 °C. Avoid repeated freeze/thaw.CYS Probe: Light sensitive. Store at -20°C. Bring to RT before use.CYS Standard: Reconstitute with 900 µl dH2O to generate 10 mM L-Cysteine Standard solution. Aliquot and Store at -20°C. Avoid repeated freeze/thaw. Use within two months.