Mitochondrial Cytochrome c Release Assay
Cytochrome c plays an important role in apoptosis. The protein is located in the space between the inner and outer mitochondrial membranes. An apoptotic stimulus triggers the release of cytochrome c from the mitochondria into cytosol where it binds to Apaf-1. The cytochrome c/Apaf-1 complex activates caspase-9, which then activates caspase-3 and other downstream caspases. The Cytochrome c Releasing Apoptosis Assay Kit provides an effective means for detecting cytochrome c translocation from mitochondria into cytosol during apoptosis. The kit provides unique formulations of reagents to isolate a highly enriched mitochondria fraction from cytosol. The procedure is so simple and easy to perform, no ultracentrifugation is required and no toxic chemicals are involved. Cytochrome c releasing from mitochondria into cytosol is then determined by Western blotting using the cytochrome c antibody provided in the kit.
Effective means for detecting cytochrome c translocation from mitochondria into cytosol during apoptosis. The kit provides an easy-to-use procedure and reagents for separating a mitochondria-enriched fraction from cytosol.
For research use only. Not intended for any diagnostic or therapeutic purpose.
Store at -20°C. Product has an expected shelf life of 12 months.
Mitochondria Extraction Buffer: 10 ml;5X Cytosol Extraction Buffer: 20 ml; DTT (1 M): 110 μL; 500X Protease Inhibitor Cocktail (Lyophilized): 1 vial-Add 250 μL DMSO to dissolve the 500X Protease Inhibitor Cocktail before use; Anti-Cytochrome c Mouse mAb (0.2 mg/ml): 0.5 ml
Compatible Sample Types:
Cells and Tissues
Features & Benefits:
1. Simple procedure; takes ~3 hours.2. Fast and convenient.3. Cytochrome c releasing from mitochondria into cytosol is then determined by Western blotting using the cytochrome c antibody provided in the kit. The procedure is simple and straightforward. No ultracentrifugation is required. The kit provides optimal reagents and antibodies for 100 assays.