Address: 45-1 Ramsey Road, Shirley, NY 11967, USA    USA: 1-631-559-9269  1-631-448-7888   Fax: 1-631-938-8127  Europe: 44-207-048-3343   Email: info@creative-biomart.com
Home > Products > Assay Kits > Activity Kits > D-Gluconate (D-Gluconic Acid) Colorimetric Assay Kit

D-Gluconate (D-Gluconic Acid) Colorimetric Assay Kit

   Share
"D-Gluconate" Related Products.
Cat.No.:
Kit-2157
Product Overview:
D-Gluconate (Gluconic Acid) Assay kit is a sensitive, fast and easy-to-use kit. In this assay, Gluconate is utilized by Gluconokinase to form D-Gluconate-6-P and ADP, which subsequently undergoes a series of reactions to form an intermediate that reduces Gluconate probe to give a product with strong absorbance at 450 nm. This assay kit can detect D-Gluconate (DGluconic Acid) level less than 2 µM in a variety of samples.
Size:
100 assays
Description:
D-Gluconic acid (C6H12O7) is a mild organic acid that is produced from glucose by glucose oxidase. It is abundantly present in plants, fruits and animal tissues. D-Gluconate (C6H11O7) is the salt or ester of Gluconic Acid. Due to its low toxicity, it is widely used in pharmaceutical, food, and other industries.
Applications:
Measurement of D-Gluconate (D-Gluconic Acid) in various samples such as tissues, plants, fruits and wine
Storage:
Store kit at -20°C, protect from light. Briefly centrifuge small vials prior to opening. Read entire protocol before performing the assay.Gluconate Assay Buffer: Warm to room temperature before use.Gluconate Converter, Gluconate Enzyme Mix, and Gluconate Developer: Reconstitute each with 220 μl Assay Buffer. Pipette up and down to dissolve completely. Aliquot and store at -20°C. Avoid repeated freeze/thaw. Keep on ice while in use. Use within two months.ATP and Gluconate Probe: Reconstitute with 220 μl dH2O. Pipette up and down to completely dissolve. Store at -20°C. Use within two months.Gluconate Standard: Reconstitute with 100 μl dH2O to generate 100 mM (100 nmol/μl) Standard solution. Store at -20°C. Use within two months. Keep on ice while in use.
Kit Components:
Gluconate Assay Buffer: 25 mlGluconate Converter: 1 vial ATP: 1 vialGluconate Enzyme Mix: 1 vialGluconate Developer: 1 vialGluconate Probe: 1 vial Gluconate Standard: 1 vial
Detection method:
Absorbance (450 nm)
Compatible Sample Types:
• Animal tissues (e.g. muscle etc.)• Wine (e.g. red wine and white wine)• Fruits (e.g. orange, apple etc.)
Features & Benefits:
• Rapid & Easy-to-use• Can measure D-Gluconate lower than 2 µM in a variety of samples


Online Inquiry    
Note: There will be extra charge for optional service!
Optional requirements on this protein +Expand
C-fusion   N-fusion  Non-tagged
His   GST  Fc  Others
<1.0 eu/μg   <0.1 eu/μg  <0.01 eu/μg  Not required
Monomer Isolation   Dimer Isolation   Not required
>80% by SDS-PAGE   >90% by SDS-PAGE  >95% by SDS-PAGE  Others
Please input "biomart" as verification code.
Please review Creative Biomart's privacy policy for more information.
Price does not include shipping and packaging costs.Request quote for final price.

If you have any other questions, please call
+1-631-559-9269.

online inquiry

Welcome! For price inquiries, please feel free to contact us through the form below. We will get back to you as soon as possible.

45-1 Ramsey Road, Shirley, NY 11967, USA
USA: 1-631-559-9269  1-631-448-7888
Europe: 44-207-048-3343
FAX: 1-631-938-8127
EMAIL: info@creative-biomart.com

facebook twitter linkedin google plus google plus

Join 10 other subscribers

24x7
CUSTOMER SERVICE
Easy access to products and services you need from our library via powerful searching tools
Copyright © 2007 - 2016 Creative Biomart. All Rights Reserved.