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Home > Products > Assay Kits > Metabolism Kits > D-Lactate Colorimetric Assay Kit

D-Lactate Colorimetric Assay Kit

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Cat.No.:
Kit-0291
Product Overview:
D-Lactate Assay Kit (Colorimetric) is used for measuring D-lactate.
Description:
D-Lactate production in mammals, mainly due to the glyoxalase pathway, is extremely low, with normal serum concentrations in the nano to micromolar range. Typically, elevated D-lactate levels which can rise to millimolar levels, are due to bacterial infection or short bowel syndrome in humans. Abnormally high concentrations of D-lactate are considered indicative of sepsis, ischemia or trauma. Due to slow metabolism and excretion, high D-lactate can cause acidosis and encephalopathy. The D-Lactate Assay Kit (Colorimetric) provides a fast, easy way to accurately measure D-lactate in a variety of biological samples. In the D-Lactate Assay Kit, D-lactate is specifically oxidized by D-lactate dehydrogenase and generates proportional color (λmax = 450 nm). The kit detects D-Lactate in samples such as serum, plasma, cells, culture and fermentation media. The useful concentration range in samples is 0.01 mM – 10 mM D-lactate.
Target Species:
Mammals
Usage:
For research use only (RUO)
Storage:
Store the kit at -20°C.
Kit Components:
D-Lactate Assay Buffer 25 mlD-Lactate Enzyme Mix: lyophilized 1 vialD-Lactate Substrate Mix: lyophilized 1 vialD-Lactate Standard (100 mM) 100 µl
Detection method:
Colorimetric
Compatible Sample Types:
Biological Fluid, Cell Culture Supernatant, Fermentation Medium, Plasma, Serum, Tissue Culture Supernatant, Urine


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Optional requirements on this protein +Expand
C-fusion   N-fusion  Non-tagged
His   GST  Fc  Others
<1.0 eu/μg   <0.1 eu/μg  <0.01 eu/μg  Not required
Monomer Isolation   Dimer Isolation   Not required
>80% by SDS-PAGE   >90% by SDS-PAGE  >95% by SDS-PAGE  Others
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45-1 Ramsey Road, Shirley, NY 11967, USA
USA: 1-631-559-9269  1-631-448-7888
Europe: 44-207-048-3343
FAX: 1-631-938-8127
EMAIL: info@creative-biomart.com

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