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Home > Products > Assay Kits > Activity Kits > DNMT Universal Chemiluminescent Assay Kit

DNMT Universal Chemiluminescent Assay Kit

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Cat.No.:
Kit-1714
Size:
96 reactions
Description:
The DNMT Universal Assay Kit is designed to measure DNMT activity for screening and profiling applications. The key to the DNMT Universal Assay Kit is a highly specific antibody that recognizes 5-methylcytosine on the substrate. With this kit, only three simple steps on a microtiter plate are required for detection of DNMT activity. First, S-adenosylmethionine is incubated with a sample containing assay buffer and DNMT for two hours. Next, primary antibody is added. Finally, the plate is treated with an HRP-labeled secondary antibody followed by addition of the HRP substrate to produce chemiluminescence that can be measured using a chemiluminescence reader.
Applications:
Useful for studying enzyme kinetics and HTS applications.
Storage:
12 months from date of receipt, when stored as directed. Kit components require different storage conditions. Be sure to store each component at the proper temperature upon arrival.
Kit Components:
DNMT1: 10 µg; -80°C DNMT3A/3L Complex: 10 µg; -80°C DNMT3B/3L Complex: 10 µg; -80°C 400 µM S-adenosylmethionine: 250 µl; -80°C Anti-5-methylcytosine antibody: 25 µl; -80°C Secondary HRP-labeled antibody 1: 10 µl; -80°C 4x DNMT assay buffer 2: 5 ml; -20°C Blocking buffer: 50 ml; +4°C HRP chemiluminescent substrate (2 components): 6 ml each; +4°C 8-well strip plate precoated with DNMT substrate: 1; +4°C


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Optional requirements on this protein +Expand
C-fusion   N-fusion  Non-tagged
His   GST  Fc  Others
<1.0 eu/μg   <0.1 eu/μg  <0.01 eu/μg  Not required
Monomer Isolation   Dimer Isolation   Not required
>80% by SDS-PAGE   >90% by SDS-PAGE  >95% by SDS-PAGE  Others
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45-1 Ramsey Road, Shirley, NY 11967, USA
USA: 1-631-559-9269  1-631-448-7888
Europe: 44-207-048-3343
FAX: 1-631-938-8127
EMAIL: info@creative-biomart.com

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