Address: 45-1 Ramsey Road, Shirley, NY 11967, USA    USA: 1-631-559-9269  1-631-448-7888   Fax: 1-631-938-8127  Europe: 44-207-048-3343   Email:
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DTNB-Thiols Assay Kit

"DTNB-Thiols" Related Products.
Product Overview:
DTNB-Thiols Assay Kit is used for the quantitiation of Sulfhydryls (DTNB-Thiols).
The DTNB-Thiols assay kit measures sulfhydryl groups with the thiol reagent 5-5dithiobis[2nitrobenzoic acid] (DTNB), which forms the 5-thionitrobenzoic acid and a mixed disulfide. Under conditioins of oxidative stress, free sulfhydryls decrease and disulfides increase. Determination of the free thiol concentration in biological samples reflects the ability to detoxify lipid and other peroxides. Exocell"s DTNB-Thiols assay can be used with a spectrum of biological samples including body fluids, tissue, and cell specimens.
For research use only (RUO)
Store this kit at 4°C. Reconstituted Ellman"s Reagent and DTNB Thiols are not stable and should be discarded after 1 day.
Kit Components:
96 well microtiter plate. 96 (8x12) wellsDTNB (Ellman"s Reagent) 1 vialEllman"s Reagent Diluent 1 vialDTNB Thiols (L-Cysteine Standard 10.0 mg vial) 1 vialDTNB Thiols Assay Diluent 1 via
Detection method:
Compatible Sample Types:
Body fluids, Cell specimens and Tissue

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Optional requirements on this protein +Expand
C-fusion   N-fusion  Non-tagged
His   GST  Fc  Others
<1.0 eu/μg   <0.1 eu/μg  <0.01 eu/μg  Not required
Monomer Isolation   Dimer Isolation   Not required
>80% by SDS-PAGE   >90% by SDS-PAGE  >95% by SDS-PAGE  Others
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45-1 Ramsey Road, Shirley, NY 11967, USA
USA: 1-631-559-9269  1-631-448-7888
Europe: 44-207-048-3343
FAX: 1-631-938-8127

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