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Home > Products > Assay Kits > Metabolism Kits > Deproteinizing Sample Preparation Kit

Deproteinizing Sample Preparation Kit

"Protein" Related Products.
Product Overview:
Deproteinizing Sample Preparation Kit is used for removing proteins from samples.
The presence of protein and various enzyme activities frequently interferes with the analysis of small molecules in biological samples. Many bioassays require removal of proteins from samples prior to analysis.Among the deproteinization protocols developed over the last half century, perchloric acid (PCA) precipitation has been extensively used in many different sample preparation procedures, since not only does it remove most of the protein present but it also functions to stabilize many of the small molecule analytes. PCA deproteinization methods have been successfully used in the preparation samples prior to quantitation of an array of small molecules, including glycogen, ATP, cAMP, glutathione, antioxidants, etc. The newly designed Deproteinizing Sample Preparation Kit utilizes a PCA precipitation method, providing a unique tool to prepare samples for the analysis of a variety of small molecules. Using the kit, proteins are precipitated, excess PCA is removed, samples are neutralized. Samples prepared using this kit can be directly used in a wide variety of bioassays. The method is easy, convenient, and can be used for the preparation of a large number of samples.
Target Species:
For research use only (RUO)
Store kit at 4°C. Keep kit components ice while in use. There may be some precipitation in the neutralization buffer. Shake bottle gently a few times to resuspend before using.
Kit Components:
PCA. Cap code: WM. 20 mlNeutralization Solution. Cap code: NM. 4 ml
Compatible Sample Types:
Cell lysate, Serum, Tissue Homogenate, Urine

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Optional requirements on this protein +Expand
C-fusion   N-fusion  Non-tagged
His   GST  Fc  Others
<1.0 eu/μg   <0.1 eu/μg  <0.01 eu/μg  Not required
Monomer Isolation   Dimer Isolation   Not required
>80% by SDS-PAGE   >90% by SDS-PAGE  >95% by SDS-PAGE  Others
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45-1 Ramsey Road, Shirley, NY 11967, USA
USA: 1-631-559-9269  1-631-448-7888
Europe: 44-207-097-1828
FAX: 1-631-938-8127

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