Address: 45-1 Ramsey Road, Shirley, NY 11967, USA    USA: 1-631-559-9269  1-631-448-7888   Fax: 1-631-938-8127  Europe: 44-207-048-3343   Email: info@creative-biomart.com
Home > Products > Assay Kits > Activity Kits > Diamine Oxidase Assay Kit

Diamine Oxidase Assay Kit

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GST(1)
His(5)
N/A(2)
Cat.No.:
Kit-2071
Product Overview:
The non-radioactive, fluorimetric DAO assay is based on the oxidation of putrescine to pyrroline plus NH3 and H2O2. The generated H2O2 is then used by HRP to oxidize a dye making it fluorescent. The increase in fluorescence at λex/em = 530/585 nm is directly proportional to the enzyme activity.
Size:
100 tests
Description:
DIAMINE OXIDASE (DAO) also known as histaminase or amine oxidase (copper containing), is an enzyme involved in the metabolism, oxidation, and inactivation of histamine in animals. Highest content is observed in the digestive tract and placenta. An imbalance between histamine intake and the capacity for histamine degradation can lead to histamine intolerance (HIT). Measuring DAO activity in serum can be useful in diagnosing HIT.
Applications:
For quantitative determination of diamine oxidase in biological samples (e.g. serum, plasma etc)
Storage:
The kit is shipped at ambient temperature. Store all components at -20°C upon receiving. Shelf life: 6 months after receipt.
Kit Components:
Assay Buffer: 10 mL HRP Enzyme: 120 µLSubstrate: 120 µL H2O2 Standard: 100 µLDye Reagent: 120 µL
Detection method:
FL530/585nm
Compatible Sample Types:
Serum, Plasma
Features & Benefits:
• Fast and sensitive. Use of 10 μL sample. Linear detection range 0.5 to 6 U/L for 30 min reaction at 25°C. • Convenient. The procedure involves adding a single working reagent, and reading the fluorescence at 0 and 30 minutes. Room temperature assay. No 37°C heater is needed.• High-throughput. "Add-mix-read" type assay. Can be readily automated as a high-throughput 96-well plate assay for thousands of samples per day.
Assay time:
30 min
Sensitivity:
Detection Limit: 0.5 U/L


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Note: There will be extra charge for optional service!
Optional requirements on this protein +Expand
C-fusion   N-fusion  Non-tagged
His   GST  Fc  Others
<1.0 eu/μg   <0.1 eu/μg  <0.01 eu/μg  Not required
Monomer Isolation   Dimer Isolation   Not required
>80% by SDS-PAGE   >90% by SDS-PAGE  >95% by SDS-PAGE  Others
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45-1 Ramsey Road, Shirley, NY 11967, USA
USA: 1-631-559-9269  1-631-448-7888
Europe: 44-207-048-3343
FAX: 1-631-938-8127
EMAIL: info@creative-biomart.com

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