Address: 45-1 Ramsey Road, Shirley, NY 11967, USA    USA: 1-631-559-9269  1-631-448-7888   Fax: 1-631-938-8127  Europe: 44-207-097-1828   Email:
Home > Products > Assay Kits > Activity Kits > Dihydrofolate Reductase Inhibitor Screening Kit

Dihydrofolate Reductase Inhibitor Screening Kit

"DHFR" Related Products.
By Species
+Expand -Collapse
By Source
+Expand -Collapse
By Tag
Product Overview:
Dihydrofolate Reductase Inhibitor Screening Kit is designed for screening DHFR inhibitors. MTX is used as a positive control. The DHFR activity is monitored by the reduction in absorbance reading at OD340 nm, while potential inhibitors arrest this decrease. The kit is adapted to a 96-well format and provides a rapid, simple, sensitive, and reliable test for high-throughput screening of DHFR inhibitors.
100 assays
Dihydrofolate Reductase (DHFR; 5,6,7,8-tetrahydrofolate NADP oxidoreductase; EC, is a ubiquitous enzyme that is present in all eukaryotic and prokaryotic cells, playing a key role in folate metabolism. Inhibition of DHFR results in reduction of the intracellular level of tetrahydrofolate, inhibition of RNA and DNA synthesis, and cell death. For this reason, DHFR has been a critically important therapeutic target for anti-tumor drugs. Methotrexate (MTX) is the most widely investigated inhibitor of DHFR, inhibiting both prokaryotic and eukaryotic DHFRs, and has shown antitumor activity.
Screening/characterizing/studying ACE2 inhibitors/activators
Upon receiving the kit, store DHFR Substrate at -80°C. Store other components at -20°C. Briefly centrifuge small vials prior to opening. Read entire protocol before performing the assay. Upon opening, use within two months.DHFR Assay Buffer: Warm to room temperature before use. Store at 4°C or -20°C.DHFR Substrate: Aliquots and store at -80°C, protect from light. Avoid repeated freeze/thaw.Dihydrofolate Reductase: Store at –20°C. Avoid repeated freeze/thaw. Keep on ice while in use.NADPH: Reconstitute with 200 μl DHFR Assay Buffer to generate NADPH Stock Solution. Aliquot and store at –20°C. Keep on ice while in use.Methotrexate: Aliquot and store at -20°C. Protect from light. Keep at room temperature while in use.
Kit Components:
DHFR Assay Buffer: 35 mlDHFR Substrate: 450 µlDihydrofolate Reductase: 30 µlNADPH: 1 vialMethotrexate (10 mM): 25 µl
Detection method:
Absorbance (OD= 340 nm)
Features & Benefits:
• Simple and reliable test to screen DHFR inhibitors/activators• High-throughput compatible• Includes Inhibitor Control (Methotrexate)

Online Inquiry    
Note: There will be extra charge for optional service!
Optional requirements on this protein +Expand
C-fusion   N-fusion  Non-tagged
His   GST  Fc  Others
<1.0 eu/μg   <0.1 eu/μg  <0.01 eu/μg  Not required
Monomer Isolation   Dimer Isolation   Not required
>80% by SDS-PAGE   >90% by SDS-PAGE  >95% by SDS-PAGE  Others
Please input "biomart" as verification code.
Please review Creative Biomart's privacy policy for more information.
Price does not include shipping and packaging costs.Request quote for final price.

If you have any other questions, please call

online inquiry

Welcome! For price inquiries, please feel free to contact us through the form below. We will get back to you as soon as possible.

45-1 Ramsey Road, Shirley, NY 11967, USA
USA: 1-631-559-9269  1-631-448-7888
Europe: 44-207-097-1828
FAX: 1-631-938-8127

facebook twitter linkedin google plus google plus

Join 10 other subscribers

Easy access to products and services you need from our library via powerful searching tools
Copyright © 2007 - 2017 Creative Biomart. All Rights Reserved.