ENO1 Human Activity Assay Kit is used to determine enolase 1 (ENO1) activity in a sample. The native enzyme is immunocaptured within the wells of the microplate; this removes all other enzymes. The enolase activity is determined via a coupled reaction to the consumption of NADH in an assay buffer.
Reacts with: HumanDoes not react withMouse, Rat
All components are shipped cold. Reagent Mix is shippedlyophilized. Store all components at 4°C. This kit is Stable for 6months from receipt.
Components 1 x 96 tests10X Blocking Buffer 1 x 6ml20X Buffer 1 x 20mlBase Buffer 1 x 24mlExtraction Buffer 1 x 15mlLDH (Lactate Dehydrogenase) 1 x 187.5 unitsMicroplate 96 antibody coated wells in 12 strips 1 unitPK (Pyruvate Kinase) 1 x 187.5 unitsReagent Mix 1 x 0.2ml
Compatible Sample Types:
Cell culture extracts, Tissue Extracts