Address: 45-1 Ramsey Road, Shirley, NY 11967, USA    USA: 1-631-559-9269  1-631-448-7888   Fax: 1-631-938-8127  Europe: 44-207-048-3343   Email:
Home > Products > Assay Kits > Signaling Kits > ERRα (human) Reporter Assay Kit
"ERRα" Related Products.
96 wells
This nuclear receptor assay system utilizes proprietary human mammalian cells engineered to provide high-level expression of a hybrid form of the human estrogen-related receptor alpha (NR3B1). The N-terminal DNA binding domains (DBD) of the native ERRα has been substituted with that of the yeast GAL4-DBD. The reporter gene is beetle luciferase functionally linked to the GAL4 upstream activation sequence (UAS). As is true in vivo, these reporter cells express ERRα in a constant state of high-level activity. The principal application of this assay system is in the screening of test samples to quantify inverse-agonist activities that they may exert against human ERRα. ERRα reporter cells are prepared using the proprietary CryoMite process. This cryo-preservation method yields exceptional cell viability post-thaw, and provides the convenience of immediately dispensing healthy, division-competent reporter cells into assay plates. There is no need for cumbersome intermediate treatment steps such as spin-and-rinse of cells, viability determinations, cell titer adjustments, or the preincubation of reporter cells prior to assay setup. The nuclear receptor reporter assays are all-inclusive cell-based assay systems. In addition to ERRα reporter cells, this kit provides two optimized media for use during cell culture and in diluting the user"s test samples, a reference inverse-agonist, reagents to prepare luciferase detection reagent, and a cell culture-ready assay plate.
Kit Components:
▪ ERα Reporter Cells: 3 x 0.60 mL; -80°C▪ Cell Recovery Media (CRM): 1 x 10.5 mL; -20°C▪ Compound Screening Media (CSM): 1 x 35 mL; -2°C ▪ XCT790, 12 mM (in DMSO) (reference agonist for ERα and ERβ): 1 x 30 µL; -20°C▪ Detection Substrate: 3 x 2.0 mL; -80°C▪ Detection Buffer:3 x 2.0 mL; -20°C ▪ Plate frame: 1; ambient ▪ Snap-in, 8-well strips (white, sterile, cell culture treated): 12; ambient

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Optional requirements on this protein +Expand
C-fusion   N-fusion  Non-tagged
His   GST  Fc  Others
<1.0 eu/μg   <0.1 eu/μg  <0.01 eu/μg  Not required
Monomer Isolation   Dimer Isolation   Not required
>80% by SDS-PAGE   >90% by SDS-PAGE  >95% by SDS-PAGE  Others
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45-1 Ramsey Road, Shirley, NY 11967, USA
USA: 1-631-559-9269  1-631-448-7888
Europe: 44-207-048-3343
FAX: 1-631-938-8127

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