Address: 45-1 Ramsey Road, Shirley, NY 11967, USA    USA: 1-631-559-9269  1-631-448-7888   Fax: 1-631-938-8127  Europe: 44-207-048-3343   Email: info@creative-biomart.com
Home > Products > Assay Kits > Activity Kits > EZH2 Homogeneous Assay Kit

EZH2 Homogeneous Assay Kit

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Cat.No.:
Kit-1727
Product Overview:
EZH2 is a histone methyltransferase that exhibits methylation activity toward H3-K27.
Size:
384 reactions
Description:
The EZH2 Homogeneous Assay Kit is designed to measure activity of the EZH2 for screening and profiling applications, using AlphaLISA technology. The key to the EZH2 Homogeneous Assay Kit is a highly specific antibody that recognizes methylated substrate. With this kit, only three simple steps on a microtiter plate are required for methyltransferase detection. First, a sample containing EZH2 enzyme is incubated with the histone substrate for one hour. Next, acceptor beads and primary antibody are added, then donor beads, followed by reading the Alpha-counts.
Applications:
Great for studying enzyme kinetics and screening small molecular inhibitors for drug discovery and HTS applications.
Storage:
12 months from date of receipt, when stored as directed. Kit components require different storage conditions. Be sure to store each component at the proper temperature upon arrival.
Kit Components:
EZH2: 100 µg; -80°C100 µM S-adenosylmethionine: 400 µl; -80°C Primary antibody 8*: 5 µl; 4°C Histone mixture (his-tagged): 400 µl; -80°C 4x HMT assay buffer 2: 3 ml; -20°C 4x Detection buffer 2: 2 ml; -20°C


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Optional requirements on this protein +Expand
C-fusion   N-fusion  Non-tagged
His   GST  Fc  Others
<1.0 eu/μg   <0.1 eu/μg  <0.01 eu/μg  Not required
Monomer Isolation   Dimer Isolation   Not required
>80% by SDS-PAGE   >90% by SDS-PAGE  >95% by SDS-PAGE  Others
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45-1 Ramsey Road, Shirley, NY 11967, USA
USA: 1-631-559-9269  1-631-448-7888
Europe: 44-207-048-3343
FAX: 1-631-938-8127
EMAIL: info@creative-biomart.com

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