Address: 45-1 Ramsey Road, Shirley, NY 11967, USA    USA: 1-631-559-9269  1-631-448-7888   Fax: 1-631-938-8127  Europe: 44-207-048-3343   Email:
Home > Products > Assay Kits > Activity Kits > EZH2 (Y641F) Chemiluminescent Assay Kit

EZH2 (Y641F) Chemiluminescent Assay Kit

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The EZH2 (Y641F) Chemiluminescent Assay Kit is designed to measure activity of the mutant EZH2 complex (EZH2 (Y641F)/EED/SUZ12/RbAp48/ AEBP) for screening and profiling purposes. The key to the EZH2 (Y641F) Chemiluminescent Assay Kit is a highly specific antibody that recognizes methylated Histone H3K27. With this kit, only three simple steps on a microtiter plate are required for methyltransferase detection. First, S-adenosylmethionine is incubated with a sample containing assay buffer and methyltransferase enzyme for one hour. Next, primary antibody is added. Finally, the strips are treated with an HRP-labeled secondary antibody followed by addition of the HRP substrate to produce chemiluminescence that can then be measured using a chemiluminescence reader.
Great for studying enzyme kinetics and HTS applications.
Stable at least 6 months from date of receipt, when stored as directed. Kit components require different storage conditions. Be sure to store each component at the proper temperature upon arrival.
Kit Components:
EZH2 (Y641F)/EED/SUZ12/RbAp48/AEBP2: 5 µg; -70°C20 µM S-adenosylmethionine: 250 µl; -70°C Primary Antibody 24: 25 µl; -70°C Secondary HRP-labeled antibody 2: 10 µl; -70°C 4x HMT assay buffer 2B: 3 ml; -20°C Blocking buffer: 50 ml; +4°C HRP chemiluminescent substrate A (translucent bottle): 6 ml; +4°C HRP chemiluminescent substrate B (brown bottle): 6 ml; +4°C 8-well strip plate module precoated with histone substrate: 1 plate (12 x 8-well strips) ; +4°C

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Optional requirements on this protein +Expand
C-fusion   N-fusion  Non-tagged
His   GST  Fc  Others
<1.0 eu/μg   <0.1 eu/μg  <0.01 eu/μg  Not required
Monomer Isolation   Dimer Isolation   Not required
>80% by SDS-PAGE   >90% by SDS-PAGE  >95% by SDS-PAGE  Others
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45-1 Ramsey Road, Shirley, NY 11967, USA
USA: 1-631-559-9269  1-631-448-7888
Europe: 44-207-048-3343
FAX: 1-631-938-8127

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