Address: 45-1 Ramsey Road, Shirley, NY 11967, USA    USA: 1-631-559-9269  1-631-448-7888   Fax: 1-631-938-8127  Europe: 44-207-048-3343   Email:
Home > Products > Assay Kits > Activity Kits > Enolase Activity Assay Kit
"Enolase" Related Products.
Product Overview:
Enolase Activity Assay Kit is a quantitative colormetric/fluorometric determination of Enolase Activity.
Enolase (EC, also called 2-phospho-D-glycerate hydrolase or 2-phosphoglycerate dehydratase, is a key enzyme in glycolysis. It converts 2-phosphoglycerate to phosphoenolpyruvate (PEP) & also catalyzes the reverse reaction, PEP to 2-phosphoglycerate under anabolic conditions during gluconeogenesis. In mammals, Enolase consists of three subunits: α, β and γ that combine into 5 isozymes, 3 of which are commonly found in human tissues. This enzyme exists in all organisms, which can undergo glycolysis. Besides its role in glycolysis, enolase has other functions too. Recent studies have shown that increased activity of enolase is associated with tumor invasion and metastasis; therefore, precise measurement of enolase activity may be of great interest for tumor diagnosis and prognosis. In Enolase Activity Assay Kit, enolase catalyzes the conversion of 2-phosphoglycerate to phosphoenolpyruvate, which is subsequently used to generate an intermediate product. The intermediate product stoichiometrically reacts with OxiRed probe to generate color (OD 570 nm) or fluorescence (Ex/Em = 535/587 nm). This simple & sensitive assay Kit can detect enolase activity less than 0.04 mU in a variety of samples.
Measurement of enolase activity in various tissues/cellsAnalysis of glycolytic metabolism in different tissues/cellsMechanistic studies of various cancersScreening anti-cancer drugs
For research use only (RUO)
Store kit at -20°C, protected from light. Warm Assay Buffer to room temperature before use. Briefly centrifuge small vials prior to opening.
Kit Components:
Enolase Assay Buffer. Cap code: WM. 25 mlOxiRed Probe (in DMSO). Cap code: red. 0.2 mlEnolase Substrate Mix (Lyophilized). Cap code: orange. 1 vialEnolase Converter (Lyophilized). Cap code: purple. 1 vialEnolase Developer (Lyophilized). Cap code: green. 1 vialEnolase Positive Control (Lyophilized). Cap code: blue. 1 vialH2O2 Standard (0.88 M). Cap code: yellow. 0.1 ml
Detection method:
Colorimetric, Fluorometric
Compatible Sample Types:
Cell Culture, Tissue

Online Inquiry    
Note: There will be extra charge for optional service!
Optional requirements on this protein +Expand
C-fusion   N-fusion  Non-tagged
His   GST  Fc  Others
<1.0 eu/μg   <0.1 eu/μg  <0.01 eu/μg  Not required
Monomer Isolation   Dimer Isolation   Not required
>80% by SDS-PAGE   >90% by SDS-PAGE  >95% by SDS-PAGE  Others
Please input "biomart" as verification code.
Please review Creative Biomart's privacy policy for more information.
Price does not include shipping and packaging costs.Request quote for final price.

If you have any other questions, please call

online inquiry

Welcome! For price inquiries, please feel free to contact us through the form below. We will get back to you as soon as possible.

45-1 Ramsey Road, Shirley, NY 11967, USA
USA: 1-631-559-9269  1-631-448-7888
Europe: 44-207-048-3343
FAX: 1-631-938-8127

facebook twitter linkedin google plus google plus

Join 10 other subscribers

Easy access to products and services you need from our library via powerful searching tools
Copyright © 2007 - 2016 Creative Biomart. All Rights Reserved.