Address: 45-1 Ramsey Road, Shirley, NY 11967, USA    USA: 1-631-559-9269  1-631-448-7888   Fax: 1-631-938-8127  Europe: 44-207-048-3343   Email: info@creative-biomart.com
Home > Products > Assay Kits > Metabolism Kits > Ferric Reducing Ascorbate Assay Kit

Ferric Reducing Ascorbate Assay Kit

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Cat.No.:
Kit-0106
Product Overview:
Ferric Reducing Ascorbate Assay Kit is used for detecting ascorbic acid.
Description:
Ascorbic Acid (Vitamin C) plays an important role in many biological processes. It is a potent anti-oxidant, anti-inflammatory, anti-viral agent and an immune stimulant and is present in a wide variety of biological specimens. Due to the presence of a variety of other antioxidants in biological samples such as serum, most ascorbic acid assays show strong interference. The Ferric Reducing Ascorbate Assay Kit provides a rapid, simple, and sensitive means of detecting ascorbic acid in biological samples such as serum and other body fluids, tissue and cell extracts, growth media and food products. In this assay, Fe+3 is reduced to Fe+2 by any antioxidants present. The ferrous iron is chelated with a colorimetric probe to produce a product with a strong absorbance band which can be monitored between 545-600 nm. The addition of ascorbate oxidase to parallel samples removes any ascorbate present leaving a background value which is subtracted from the total to give ascorbate content. The assay can detect 0.2 to 20 nmol of ascorbic acid in various samples.
Target Species:
Mammals
Usage:
For research use only (RUO)
Storage:
Store kit at -20°C, protect from light. Warm FRASC Buffer to room temperature before use. Briefly centrifuge all small vials prior to opening. Make sure the plate reader is turned on and ready to use.
Kit Components:
FRASC Buffer 25 mLAscorbic Acid Probe 1 mLFeCl3 solution 1 mLAscorbate Oxidase (lyophilized) 1 vialAscorbic Acid Standard (20 µmole) 1 vial
Detection method:
Colorimetric
Compatible Sample Types:
Biological Fluid, Cell Culture Supernatant, Food Sample, Growth Medium, Plasma, Serum, Tissue Culture Supernatant, Urine


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Optional requirements on this protein +Expand
C-fusion   N-fusion  Non-tagged
His   GST  Fc  Others
<1.0 eu/μg   <0.1 eu/μg  <0.01 eu/μg  Not required
Monomer Isolation   Dimer Isolation   Not required
>80% by SDS-PAGE   >90% by SDS-PAGE  >95% by SDS-PAGE  Others
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45-1 Ramsey Road, Shirley, NY 11967, USA
USA: 1-631-559-9269  1-631-448-7888
Europe: 44-207-048-3343
FAX: 1-631-938-8127
EMAIL: info@creative-biomart.com

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