RTK Activity cell lines are engineered to a fusion of the intracellular catalytic domain from the target RTK and the extracellular ligand binding domain of EGFR. This fusion protein is tagged with ProLink (PK). The cells co-an Enzyme Acceptor (EA) tagged SH2 domain. Ligand induced cross-phosphorylation of the PK-tagged fusion protein leads to SH2-EA recruitment, forcing complementation of the two β-galactosidase enzyme fragments (EA and PK). The resulting functional enzyme hydrolyzes substrate to generate a chemiluminescent signal. These cells have been modified to prevent long term propagation and expansion using a proprietary compound that has no apparent effect on assay performance.
100 dp(1 x 96-well)/200 dp(2 x 96-well)/1000 dp(10 x 96-well)
Short term (2 weeks): Store in vapor phase of liquid N2
1.2 x 10^6 cells per vial in 0.1 mL (kit contains cells, detection reagents and plate(s))