Free Glycerol Assay Kit is used for measuring free glycerol concentration.
Glycerol is widely used in foods, beverages, solvents, pharmaceutical and cosmetic products, etc. There is broad interest in quantification of glycerol for research and development. The Free Glycerol Assay Kit provides a sensitive, easy assay to measure free glycerol concentration in various samples. In the assay, glycerol is enzymatically oxidized to generate a product which reacts with the probe to generate color (λ = 570 nm) and fluorescence (Ex/Em = 535/587 nm). The kit can detect 50 pmol-10 nmol (or 1~10000 μM range) of glycerol in various samples.
For research use only (RUO)
Store kit at -20°C, protect from light. Warm Assay Buffer to room temperature before use. Briefly centrifuge all small vials prior to opening.
Glycerol Assay Buffer 25 mLGlycerol Probe (in DMSO, Anhydrous) 0.2 mLGlycerol Enzyme Mix (lyophilized) 1 vialGlycerol Standard (100 mM) 0.2 mL
Compatible Sample Types:
Biological Fluid, Cell Culture Supernatant, Plasma, Serum, Tissue Culture Supernatant, Urine