Address: 45-1 Ramsey Road, Shirley, NY 11967, USA    USA: 1-631-559-9269  1-631-448-7888   Fax: 1-631-938-8127  Europe: 44-207-048-3343   Email:
Home > Products > Assay Kits > Signaling Kits > Fura-2 No Wash Calcium Assay Kit
"Fura-2" Related Products.
Product Overview:
Fura-2 No Wash Calcium Assay Kit is a ratiometric calcium assay that allows homogeneous measurement of intracellular calcium mobilization caused by activation of G-protein-coupled receptors (GPCR) or calcium channels. Cells expressing a GPCR of interest that signals through calcium are pre-loaded with Fura-2 AM which can cross cell membrane. Once inside the cell, the lipophilic blocking groups of Fura-2 AM are cleaved by esterases, resulting in a negatively charged fluorescent dye that stays inside cells and its fluorescence wavelength is blue-shifted upon binding to calcium. When cells are stimulated with agonists, the receptor signals the release of intracellular calcium, which greatly increase the fluorescence intensity of Fura-2 at the short wavelength. The characteristics of its ratio measurements reduce the effects of uneven dye loading and cell numbers, dye leakage and photo bleaching. The 340/380 nm excitation ratio allows accurate measurements of the intracellular Ca2+ concentration and makes this kit an ideal tool for more accurate measurement of cellular calcium concentration compared to Fluo-8 of the single wavelength. The kit provides an optimized assay method with no washing step for monitoring the G-protein-coupled receptors and calcium channels using HTS instrumentation. The assay can be performed in a convenient 96-well or 384-well microtiter-plate format.
10 x 96 tests
This product is intended to be used for monitoring calcium fluctuations in vivo in live cells using the following HTS imaging plate readers: FLIPR, FDSS, BMG NOVOstar, FLexStation, ViewLux, IN Cell Analyzer or Arrayscan.
Functional Studies more details
Target Species:
Reacts with: Human, Chinese Hamster; Predicted to work with: all Mammals
Store at -20°C.
Kit Components:
Components: Identifier; 10X Pluronic F127 Plus; Fura-2 AM; HHBS
Detection method:
Compatible Sample Types:
Adherent cells, Suspension cells

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Optional requirements on this protein +Expand
C-fusion   N-fusion  Non-tagged
His   GST  Fc  Others
<1.0 eu/μg   <0.1 eu/μg  <0.01 eu/μg  Not required
Monomer Isolation   Dimer Isolation   Not required
>80% by SDS-PAGE   >90% by SDS-PAGE  >95% by SDS-PAGE  Others
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45-1 Ramsey Road, Shirley, NY 11967, USA
USA: 1-631-559-9269  1-631-448-7888
Europe: 44-207-048-3343
FAX: 1-631-938-8127

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