Address: 45-1 Ramsey Road, Shirley, NY 11967, USA    USA: 1-631-559-9269  1-631-448-7888   Fax: 1-631-938-8127  Europe: 44-207-048-3343   Email: info@creative-biomart.com
Home > Products > Assay Kits > Cellular Kits > G-Actin/F-actin In Vivo Assay Biochem Kit

G-Actin/F-actin In Vivo Assay Biochem Kit

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Cat.No.:
Kit-0354
Product Overview:
The most reproducible and accurate method of determining the amount of filamentous actin (F-actin) content versus free globular-actin (G-actin) content in a cell population is to use Western blot quantitation of F-actin and G-actin cellular fractions. The general approach is to homogenize cells in F-actin stabilization buffer, followed by centrifugation to separate the F-actin from G-actin pool. The fractions are then separated by SDS-PAGE and actin is quantitated by Western blot. The final result gives the most accurate method of determining the ratio of F-actin incorporated into the cytoskeleton versus the G-actin found in the cytosol. This kit contains all the reagents needed to perform this assay.
Size:
30-100 assays
Applications:
1. To study the effects of pharmaceutical compounds on the ratio of G-actin to F-actin.2. To study the effects of mutated cell lines versus their parent cell line for the change in ratio of G-actin to F-actin.3. To study the effects of physical alterations of environment on the ratio of G-actin to F-actin.
Kit Components:
Reagent-Quantity -StorageLysis and F-actin Stabilzation Buffer: 1 bottle, 100 ml, 4°C; Protease Inhibitor Cocktail: 1 tube, lyophilized, Desiccated 4°C; ATP: 1 tube, lyophilized, Desiccated 4°C; F-actin Depolymerizing Buffer: 1 bottle, powder, 4°C;F-actin Enhancing Solution: 1 tube, lyophilized, Desiccated 4°C G-actin Protein Standard: 1 tube, 250 µg, lyophilized, Desiccated 4°C; Anti-Actin Rabbit Polyclonal Antibody: 1 tube, 100 µg lyophilized, Desiccated 4°C; SDS Sample Buffer: 2 tubes, 1.5 ml, 5x stock, 4°C; DMSO: 2 tubes, 1 ml, 4°C


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Optional requirements on this protein +Expand
C-fusion   N-fusion  Non-tagged
His   GST  Fc  Others
<1.0 eu/μg   <0.1 eu/μg  <0.01 eu/μg  Not required
Monomer Isolation   Dimer Isolation   Not required
>80% by SDS-PAGE   >90% by SDS-PAGE  >95% by SDS-PAGE  Others
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45-1 Ramsey Road, Shirley, NY 11967, USA
USA: 1-631-559-9269  1-631-448-7888
Europe: 44-207-048-3343
FAX: 1-631-938-8127
EMAIL: info@creative-biomart.com

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