|Product Overview:||Recombinant Human CSF2, Ala18-Glu144, was produced in E. coli using non-animal reagents in an animal-free facility under cGMP guidelines.|
|Form:||Lyophilized from a 0.2 μm filtered solution in PBS.|
|Bio-activity:||Measured in a cell proliferation assay using TF‑1 human erythroleukemic cells. Kitamura, T. et al. (1989) J. Cell Physiol. 140:323. The ED50 for this effect is 6-30 pg/mL.
The specific activity of recombinant human GM-CSF is approximately 1.5 x 10^4 IU/μg, which is calibrated against human GM-CSF WHO International Standard (NIBSC code: 88/646).
|Molecular Mass:||Predicted Molecular Mass: 14.5 kDa
SDS-PAGE: 14 kDa, reducing conditions
|N-terminal Sequence Analysis:||Ala-Pro-Ala-Arg-Ser-Pro-Ser-Pro-Ser-Thr|
|Endotoxin:||<0.10 EU per 1 μg of the protein by the LAL method.|
|Purity:||>97%, by SDS-PAGE under reducing conditions and visualized by silver stain.|
|Storage:||Avoid repeated freeze-thaw cycles.
12 months, -20 to -70 centigrade as supplied.
1 month, 2 to 8 centigrade under sterile conditions after reconstitution.
3 months, -20 to -70 centigrade under sterile conditions after reconstitution.
|Reconstitution:||Reconstitute at 100-200 μg/mL in PBS.|
|Quality Statement:||Our GMP proteins are produced according to relevant sections of the following documents: WHO TRS, No. 822, 1992 Annex 1, Good Manufacturing Practices for Biological Products; USP Chapter 1043, Ancillary Materials for Cell, Gene and Tissue-Engineered Products and USP Chapter 92, Growth Factors and Cytokines Used in Cell Therapy Manufacturing.|
|Shipping:||The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature.|
|Gene Name:||CSF2 colony stimulating factor 2 (granulocyte-macrophage) [ Homo sapiens ]|
|Synonyms:||CSF2; GM CSF; GMCSF; granulocyte macrophage colony stimulating factor; molgramostin; sargramostim; CSF; colony-stimulating factor;|
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