GMP Recombinant Human EGF Protein

Cat.No.: EGF-100HG
Product Overview: Recombinant Human EGF, Asn971-Arg1023, was produced in E. coli using non-animal reagents in an animal-free facility under cGMP guidelines.
Source: E. coli
Species: Human
Form: Lyophilized from a 0.2 μm filtered solution in PBS.
Bio-activity: Measured in a cell proliferation assay using Balb/3T3 mouse embryonic fibroblast cells. Rubin, J.S. et al. (1991) Proc. Natl. Acad. Sci. USA 88:415. The ED50 for this effect is 20‑100 pg/mL.
The specific activity of Recombinant Human EGF is approximately 1.4 x 10^3 IU/μg, which is calibrated against human EGF WHO International Standard (NIBSC code: 91/530).
Molecular Mass: Predicted Molecular Mass: 6 kDa
SDS-PAGE: 6 kDa, reducing conditions
N-terminal Sequence Analysis: Met-Asn971-Ser-Asp-Ser-Glu-(Cys)-Pro-Leu-Ser
Endotoxin: <0.10 EU per 1 μg of the protein by the LAL method.
Purity: >97%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Storage: Avoid repeated freeze-thaw cycles.
12 months, -20 to -70 centigrade as supplied.
1 month, 2 to 8 centigrade under sterile conditions after reconstitution.
3 months, -20 to -70 centigrade under sterile conditions after reconstitution.
Reconstitution: Reconstitute at 200 μg/mL in PBS.
Quality Statement: Our GMP proteins are produced according to relevant sections of the following documents: WHO TRS, No. 822, 1992 Annex 1, Good Manufacturing Practices for Biological Products; USP Chapter 1043, Ancillary Materials for Cell, Gene and Tissue-Engineered Products and USP Chapter 92, Growth Factors and Cytokines Used in Cell Therapy Manufacturing.
Shipping: The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature.
Gene Name: EGF epidermal growth factor [ Homo sapiens ]
Official Symbol: EGF
Synonyms: EGF; epidermal growth factor; epidermal growth factor (beta urogastrone); pro-epidermal growth factor; beta-urogastrone; URG; HOMG4;
Gene ID: 1950
mRNA Refseq: NM_001178130
Protein Refseq: NP_001171601
MIM: 131530
UniProt ID: P01133

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Optional requirements on this protein    +Expand
C-fusion    N-fusion   Non-tagged
His    GST   Fc   Others
<1.0 eu/μg    <0.1 eu/μg   <0.01 eu/μg   Not required
Monomer Isolation    Dimer Isolation    Not required
>80% by SDS-PAGE    >90% by SDS-PAGE   >95% by SDS-PAGE   Others

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