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Active GMP Recombinant Human FN1 Protein

Cat.No. : FN1-154HG
Product Overview : Recombinant Human NF1, Pro1270-Ser1546&Ala1721-Thr2016, was produced in E. coli using non-animal reagents in an animal-free facility under cGMP guidelines.
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  • Gene Information
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Description : Fibronectin1(FN1) is a secreted protein and contains 12 fibronectin type-I domains,fibronectin type-II domains and 16 fibronectin type-III domains.Recombinant human fibronectin fragment, is a protein of ~63 kDa containing a central cell-binding domain, a high affinity heparin-binding domain II,and CS1 site within the alternatively spliced III CS region of human fibronectin. Cells bind to a VLA-4 ligand, a CS-I site, and a VLA-5 ligand, a cell attachment domain, and virus vectors binds to a heparin binding domain II, which co-locates the cell and the virus vector on NovoNectin. This process enhances the density of both cells and vectors, and facilitates the gene transduction in the result.
Source : E. coli
Species : Human
Form : Lyophilized.
Bio-activity : Measured by the ability of the immobilized protein to support the adhesion of Jurkat human acute T cell leukemia cells.
When 1×10E5 cells/well areadded to Fibronectin-coated plates (7-13 ng/mL and 100μL/well), approximately 50%-80% will adhere specifically after 30 minutes at 37°C.
AA Sequence : MPTDLRFTNIGPDTMRVTWAPPPSI DLTNFLVRYSPVKNEEDVAELSISP SDNAVVLTNLLPGTE YVVSVSSVYEQHESTPLRGRQKTGL DSPTGIDFSDITANSFTVHWIAPRA TITGYRIRHHPEHFS GRPREDRVPHSRNSITLTNLTPGTE YVVSIVALNGREESPLLIGQQSTVS DVPRDLEVVAATPTS LLISWDAPAVTVRYYRITYGETGGN SPVQEFTVPGSKSTATISGLKPGVD YTITVYAVTGRGDSP ASSKPISINYRTEIDKPSMAIPAPT DLKFTQVTPTSLSAQWTPPNVQLTG YRVRVTPKEKTGPMK EINLAPDSSSVVVSGLMVATKYEVS VYALKDTLTSRPAQGVVTTLENVSP PRRARVTDATETTIT ISWRTKTETITGFQVDAVPANGQTP IQRTIKPDVRSYTITGLQPGTDYKI YLYTLNDNARSSPVV IDASTAIDAPSNLRFLATTPNSLLV SWQPPRARITGYIIKYEKPGSPPRE VVPRPRPGVTEATIT GLEPGTEYTIYVIALKNNQKSEPLI GRKKTDELPQLVTLPHPNLHGPEIL DVPST
Residual Host Cell DNA Content : <10pg/mg
Residual Host Cell Protein Content : <1ug/mg
Endotoxin : <0.1EU/ug
Purity : Greater than 95% as determined by reducing SDS-PAGE.
Storage : Lyophilized and reconstituted protein should be stored at -80 centigrade. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
Reconstitution : Always centrifuge tubes before opening. Do not mix by vortex or pipetting.
It is not recommended to reconstitute to a concentration >100μg/ml.
Dissolve the lyophilized protein in ddH2O.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
Quality Statement : Our GMP proteins are produced according to relevant sections of the following documents: WHO TRS, No. 822, 1992 Annex 1, Good Manufacturing Practices for Biological Products; USP Chapter 1043, Ancillary Materials for Cell, Gene and Tissue-Engineered Products and USP Chapter 92, Growth Factors and Cytokines Used in Cell Therapy Manufacturing.
Shipping : The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature.
Gene Name : FN1 fibronectin 1 [ Homo sapiens ]
Official Symbol : FN1
Synonyms : FN1; fibronectin 1; fibronectin; CIG; cold insoluble globulin; FINC; GFND2; LETS; migration stimulating factor; MSF; cold-insoluble globulin; migration-stimulating factor; FN; FNZ; ED-B; GFND
Gene ID : 2335
mRNA Refseq : NM_002026
Protein Refseq : NP_002017
MIM : 135600
UniProt ID : P02751

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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Q&As (6)

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which organs and tissues is the FN1 protein expressed? 12/21/2019

It is expressed in a variety of organs and tissues, including skin, kidneys, cardiovascular system, liver, etc.

How about the FN1 protein in inflammatory diseases? 09/09/2019

FN1 protein is involved in the migration, adhesion and activation of inflammatory cells during the inflammatory response, and plays an important role in the development of inflammation.

Have regulators of the FN1 protein been identified? 07/16/2019

Yes, multiple factors have been found to regulate the expression and function of FN1 protein, including transcription factors, growth factors, etc.

What is the relationship between FN1 protein and tumor metastasis? 06/26/2019

FN1 protein is involved in the invasion and metastasis of tumor cells, which can provide matrix support and guide signals to promote the migration and invasion of tumor cells.

What is the role of FN1 protein in wound healing? 04/15/2019

This protein is involved in key steps such as fibrosis, angiogenesis and interstitial cell proliferation in the wound healing process, and plays an important role in wound repair.

How about the mechanism of action of FN1 protein in tissue repair? 04/13/2019

The protein plays an important role in key steps such as extracellular matrix remodeling, cell adhesion, and directed differentiation of mesenchymal stem cells during tissue repair.

Customer Reviews (3)

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Reviews
06/21/2021

    The preservation conditions are wide and the efficiency is high.

    08/14/2019

      Experimental process is convenient, which greatly improves the efficiency of scientific research.

      03/25/2019

        Biological activity of this protein is amazing, and you can see that its production process is very pure, which has a crucial effect on the quality of the product.

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