Active GMP Recombinant Human FN1 Protein
Cat.No. : | FN1-154HG |
Product Overview : | Recombinant Human NF1, Pro1270-Ser1546&Ala1721-Thr2016, was produced in E. coli using non-animal reagents in an animal-free facility under cGMP guidelines. |
- Specification
- Gene Information
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Description : | Fibronectin1(FN1) is a secreted protein and contains 12 fibronectin type-I domains,fibronectin type-II domains and 16 fibronectin type-III domains.Recombinant human fibronectin fragment, is a protein of ~63 kDa containing a central cell-binding domain, a high affinity heparin-binding domain II,and CS1 site within the alternatively spliced III CS region of human fibronectin. Cells bind to a VLA-4 ligand, a CS-I site, and a VLA-5 ligand, a cell attachment domain, and virus vectors binds to a heparin binding domain II, which co-locates the cell and the virus vector on NovoNectin. This process enhances the density of both cells and vectors, and facilitates the gene transduction in the result. |
Source : | E. coli |
Species : | Human |
Form : | Lyophilized. |
Bio-activity : | Measured by the ability of the immobilized protein to support the adhesion of Jurkat human acute T cell leukemia cells. When 1×10E5 cells/well areadded to Fibronectin-coated plates (7-13 ng/mL and 100μL/well), approximately 50%-80% will adhere specifically after 30 minutes at 37°C. |
AA Sequence : | MPTDLRFTNIGPDTMRVTWAPPPSI DLTNFLVRYSPVKNEEDVAELSISP SDNAVVLTNLLPGTE YVVSVSSVYEQHESTPLRGRQKTGL DSPTGIDFSDITANSFTVHWIAPRA TITGYRIRHHPEHFS GRPREDRVPHSRNSITLTNLTPGTE YVVSIVALNGREESPLLIGQQSTVS DVPRDLEVVAATPTS LLISWDAPAVTVRYYRITYGETGGN SPVQEFTVPGSKSTATISGLKPGVD YTITVYAVTGRGDSP ASSKPISINYRTEIDKPSMAIPAPT DLKFTQVTPTSLSAQWTPPNVQLTG YRVRVTPKEKTGPMK EINLAPDSSSVVVSGLMVATKYEVS VYALKDTLTSRPAQGVVTTLENVSP PRRARVTDATETTIT ISWRTKTETITGFQVDAVPANGQTP IQRTIKPDVRSYTITGLQPGTDYKI YLYTLNDNARSSPVV IDASTAIDAPSNLRFLATTPNSLLV SWQPPRARITGYIIKYEKPGSPPRE VVPRPRPGVTEATIT GLEPGTEYTIYVIALKNNQKSEPLI GRKKTDELPQLVTLPHPNLHGPEIL DVPST |
Residual Host Cell DNA Content : | <10pg/mg |
Residual Host Cell Protein Content : | <1ug/mg |
Endotoxin : | <0.1EU/ug |
Purity : | Greater than 95% as determined by reducing SDS-PAGE. |
Storage : | Lyophilized and reconstituted protein should be stored at -80 centigrade. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
Reconstitution : | Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration >100μg/ml. Dissolve the lyophilized protein in ddH2O. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
Quality Statement : | Our GMP proteins are produced according to relevant sections of the following documents: WHO TRS, No. 822, 1992 Annex 1, Good Manufacturing Practices for Biological Products; USP Chapter 1043, Ancillary Materials for Cell, Gene and Tissue-Engineered Products and USP Chapter 92, Growth Factors and Cytokines Used in Cell Therapy Manufacturing. |
Shipping : | The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature. |
Gene Name : | FN1 fibronectin 1 [ Homo sapiens ] |
Official Symbol : | FN1 |
Synonyms : | FN1; fibronectin 1; fibronectin; CIG; cold insoluble globulin; FINC; GFND2; LETS; migration stimulating factor; MSF; cold-insoluble globulin; migration-stimulating factor; FN; FNZ; ED-B; GFND |
Gene ID : | 2335 |
mRNA Refseq : | NM_002026 |
Protein Refseq : | NP_002017 |
MIM : | 135600 |
UniProt ID : | P02751 |
Products Types
◆ Recombinant Protein | ||
FN1-3296M | Recombinant Mouse FN1 Protein, His (Fc)-Avi-tagged | +Inquiry |
FN1-01H | Recombinant Human FN1 Protein, T7-His-TEV-tagged | +Inquiry |
FN1-938H | Recombinant Human FN1 Protein, His-tagged | +Inquiry |
FN1-2741P | Recombinant Pig FN1 Protein, His-tagged | +Inquiry |
FN1-4400H | Recombinant Human FN1 Protein, GST-tagged | +Inquiry |
◆ Native Protein | ||
FN1-700H | Native Human Fibronectin 1 | +Inquiry |
FN1-701H | Native Human Fibronectin 1 | +Inquiry |
FN1-4399H | Native Human FN1 Protein | +Inquiry |
◆ Lysates | ||
FN1-2956HCL | Recombinant Human FN1 cell lysate | +Inquiry |
Related Gene
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
Inquiry
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Q&As (6)
Ask a questionIt is expressed in a variety of organs and tissues, including skin, kidneys, cardiovascular system, liver, etc.
FN1 protein is involved in the migration, adhesion and activation of inflammatory cells during the inflammatory response, and plays an important role in the development of inflammation.
Yes, multiple factors have been found to regulate the expression and function of FN1 protein, including transcription factors, growth factors, etc.
FN1 protein is involved in the invasion and metastasis of tumor cells, which can provide matrix support and guide signals to promote the migration and invasion of tumor cells.
This protein is involved in key steps such as fibrosis, angiogenesis and interstitial cell proliferation in the wound healing process, and plays an important role in wound repair.
The protein plays an important role in key steps such as extracellular matrix remodeling, cell adhesion, and directed differentiation of mesenchymal stem cells during tissue repair.
Customer Reviews (3)
Write a reviewThe preservation conditions are wide and the efficiency is high.
Experimental process is convenient, which greatly improves the efficiency of scientific research.
Biological activity of this protein is amazing, and you can see that its production process is very pure, which has a crucial effect on the quality of the product.
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