|Product Overview:||Recombinant Human HGF, Gln32-Ser728, was produced in NS0 cell under cGMP guidelines.|
|Form:||Lyophilized from a 0.2 μm filtered solution in PBS.|
|Bio-activity:||Measured by its ability to induce IL-11 secretion by Saos‑2 human osteosarcoma cells. Hjertner, O. et al. (1999) Blood 94:3883.
The specific activity of recombinant human HGF is approximately 1.5 x 10^3 IU/μg, which is calibrated against recombinant human HGF WHO International Standard (NIBSC code: 96/564).
|Molecular Mass:||Predicted Molecular Mass: alpha chain: 53.7; beta chain: 26 kDa
SDS-PAGE: 65-75 kDa, non-reducing conditions
60-65 kDa, and 30-40 kDa, reducing conditions
|N-terminal Sequence Analysis:||Amino acid sequencing of the alpha chain was blocked, suggesting it is consistent with Gln32
Gln32-Arg-Lys-Arg-Arg-Asn-Thr-Ile-His-Glu (alpha -chain)
Val495-Val-Asn-Gly-Ile-Pro-Thr-Arg-Thr-Asn (beta -chain)
|Endotoxin:||<0.10 EU per 1 μg of the protein by the LAL method.|
|Purity:||>95%, by SDS-PAGE with silver staining, under reducing conditions.|
|Storage:||Avoid repeated freeze-thaw cycles.
12 months, -20 to -70 centigrade as supplied.
1 month, 2 to 8 centigrade under sterile conditions after reconstitution.
3 months, -20 to -70 centigrade under sterile conditions after reconstitution.
|Reconstitution:||Reconstitute at 100 μg/mL in PBS.|
|Quality Statement:||Our GMP proteins are produced according to relevant sections of the following documents: WHO TRS, No. 822, 1992 Annex 1, Good Manufacturing Practices for Biological Products; USP Chapter 1043, Ancillary Materials for Cell, Gene and Tissue-Engineered Products and USP Chapter 92, Growth Factors and Cytokines Used in Cell Therapy Manufacturing.|
|Shipping:||The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature.|
|Gene Name:||HGF hepatocyte growth factor (hepapoietin A; scatter factor) [ Homo sapiens ]|
|Synonyms:||HGF; hepatocyte growth factor; F TCF; fibroblast derived tumor cytotoxic factor; hepatopoietin A; HGFB; HPTA; scatter factor; SF; hepatopoeitin-A; hepatopoietin-A; F-TCF; DFNB39;|
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