Active GMP Recombinant Human HGF Protein

• Cat.No.: HGF-143HG
• Product Overview: Recombinant Human HGF, Gln32-Ser728, was produced in NS0 cell under cGMP guidelines.

Specification

• Source: Mammalian cells
• Species: Human
• Form: Lyophilized from a 0.2 μm filtered solution in PBS.
• Bio-activity: Measured by its ability to induce IL-11 secretion by Saos‑2 human osteosarcoma cells. Hjertner, O. et al. (1999) Blood 94:3883.
  The specific activity of recombinant human HGF is approximately 1.5 x 10^3 IU/μg, which is calibrated against recombinant human HGF WHO International Standard (NIBSC code: 96/564).
• Molecular Mass: Predicted Molecular Mass: alpha chain: 53.7; beta chain: 26 kDa
  SDS-PAGE: 65-75 kDa, non-reducing conditions
  60-65 kDa, and 30-40 kDa, reducing conditions
• N-terminal Sequence Analysis: Amino acid sequencing of the alpha chain was blocked, suggesting it is consistent with Gln32
  Gln32-Arg-Lys-Arg-Arg-Asn-Thr-Ile-His-Glu (alpha -chain)
  Val495-Val-Asn-Gly-Ile-Pro-Thr-Arg-Thr-Asn (beta -chain)
• Endotoxin: <0.10 EU per 1 μg of the protein by the LAL method.
• Purity: >95%, by SDS-PAGE with silver staining, under reducing conditions.
• Storage: Avoid repeated freeze-thaw cycles.
  12 months, -20 to -70 centigrade as supplied.
  1 month, 2 to 8 centigrade under sterile conditions after reconstitution.
  3 months, -20 to -70 centigrade under sterile conditions after reconstitution.
• Reconstitution: Reconstitute at 100 μg/mL in PBS.
• Quality Statement: Our GMP proteins are produced according to relevant sections of the following documents: WHO TRS, No. 822, 1992 Annex 1, Good Manufacturing Practices for Biological Products; USP Chapter 1043, Ancillary Materials for Cell, Gene and Tissue-Engineered Products and USP Chapter 92, Growth Factors and Cytokines Used in Cell Therapy Manufacturing.
• Shipping: The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature.

Gene Information

• Gene Name: HGF hepatocyte growth factor (hepapoietin A; scatter factor) [ Homo sapiens ]
• Official Symbol: HGF
• Synonyms: HGF; hepatocyte growth factor; F TCF; fibroblast derived tumor cytotoxic factor; hepatopoietin A; HGFB; HPTA; scatter factor; SF; hepatopoeitin-A; hepatopoietin-A; F-TCF; DFNB39
• Gene ID: 3082
• mRNA Refseq: NM_000601
• Protein Refseq: NP_000592
• MIM: 142409
• UniProt ID: P14210