Address: 45-1 Ramsey Road, Shirley, NY 11967, USA    USA: 1-631-559-9269  1-631-448-7888   Fax: 1-631-938-8127  Europe: 44-207-097-1828   Email:
Home > Products > GMP Proteins > GMP Recombinant Human HGF Protein

GMP Recombinant Human HGF Protein

"HGF" Related Products.
Product Overview:
Recombinant Human HGF, Gln32-Ser728, was produced in NS0 cell under cGMP guidelines.
Lyophilized from a 0.2 μm filtered solution in PBS.
Measured by its ability to induce IL-11 secretion by Saos‑2 human osteosarcoma cells. Hjertner, O. et al. (1999) Blood 94:3883.
The specific activity of recombinant human HGF is approximately 1.5 x 10^3 IU/μg, which is calibrated against recombinant human HGF WHO International Standard (NIBSC code: 96/564).
Molecular Mass:
Predicted Molecular Mass: alpha chain: 53.7; beta chain: 26 kDa
SDS-PAGE: 65-75 kDa, non-reducing conditions
60-65 kDa, and 30-40 kDa, reducing conditions
N-terminal Sequence Analysis:
Amino acid sequencing of the alpha chain was blocked, suggesting it is consistent with Gln32
Gln32-Arg-Lys-Arg-Arg-Asn-Thr-Ile-His-Glu (alpha -chain)
Val495-Val-Asn-Gly-Ile-Pro-Thr-Arg-Thr-Asn (beta -chain)
<0.10 EU per 1 μg of the protein by the LAL method.
>95%, by SDS-PAGE with silver staining, under reducing conditions.
Avoid repeated freeze-thaw cycles.
12 months, -20 to -70 centigrade as supplied.
1 month, 2 to 8 centigrade under sterile conditions after reconstitution.
3 months, -20 to -70 centigrade under sterile conditions after reconstitution.
Reconstitute at 100 μg/mL in PBS.
Quality Statement:
Our GMP proteins are produced according to relevant sections of the following documents: WHO TRS, No. 822, 1992 Annex 1, Good Manufacturing Practices for Biological Products; USP Chapter 1043, Ancillary Materials for Cell, Gene and Tissue-Engineered Products and USP Chapter 92, Growth Factors and Cytokines Used in Cell Therapy Manufacturing.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature.

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Optional requirements on this protein +Expand
C-fusion   N-fusion  Non-tagged
His   GST  Fc  Others
<1.0 eu/μg   <0.1 eu/μg  <0.01 eu/μg  Not required
Monomer Isolation   Dimer Isolation   Not required
>80% by SDS-PAGE   >90% by SDS-PAGE  >95% by SDS-PAGE  Others
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45-1 Ramsey Road, Shirley, NY 11967, USA
USA: 1-631-559-9269  1-631-448-7888
Europe: 44-207-097-1828
FAX: 1-631-938-8127

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