Address: 45-1 Ramsey Road, Shirley, NY 11967, USA    USA: 1-631-559-9269  1-631-448-7888   Fax: 1-631-938-8127  Europe: 44-207-048-3343   Email: info@creative-biomart.com
Home > Products > GMP Proteins > GMP Recombinant Human IFNG Protein

GMP Recombinant Human IFNG Protein

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Fc(2)
Fc-His(1)
His(27)
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Cat.No.:
IFNG-103HG
Product Overview:
Recombinant Human IFNG, Gln24-Gln166, was produced in E. coli using non-animal reagents in an animal-free facility under cGMP guidelines.
Source:
E. coli
Species:
Human
Form:
Lyophilized from a 0.2 μm filtered solution in Sodium Succinate, Mannitol and Tween® 80.
Bio-activity:
Measured in anti-viral assays using HeLa human cervical epithelial carcinoma cells infected with encephalomyocarditis (EMC) virus. Meager, A. (1987) in Lymphokines and Interferons, a Practical Approach. Clemens, M.J. et al. (eds): IRL Press. 129. The ED50 for this effect is 0.15-0.75 ng/mL.
The specific activity of recombinant human IFN-gamma is approximately 2 x 10^4 IU/μg, which is calibrated against human IFN-gamma Standard (NIBSC code: 87/586).
Molecular Mass:
Predicted Molecular Mass: 16.9 kDa
SDS-PAGE: 17 kDa, reducing conditions
N-terminal Sequence Analysis:
Met-Gln24-Asp-Pro-Tyr-Val-Lys-Glu-Ala-Glu
Endotoxin:
<0.01 EU per 1 μg of the protein by the LAL method.
Purity:
>97%, by SDS-PAGE with silver staining, under reducing
Storage:
Avoid repeated freeze-thaw cycles.
12 months, -20 to -70 centigrade as supplied.
1 month, 2 to 8 centigrade under sterile conditions after reconstitution.
3 months, -20 to -70 centigrade under sterile conditions after reconstitution.
Reconstitution:
Reconstitute at 200 μg/mL in sterile, deionized water.
Quality Statement:
Our GMP proteins are produced according to relevant sections of the following documents: WHO TRS, No. 822, 1992 Annex 1, Good Manufacturing Practices for Biological Products; USP Chapter 1043, Ancillary Materials for Cell, Gene and Tissue-Engineered Products and USP Chapter 92, Growth Factors and Cytokines Used in Cell Therapy Manufacturing.
Shipping:
The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature.


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Optional requirements on this protein +Expand
C-fusion   N-fusion  Non-tagged
His   GST  Fc  Others
<1.0 eu/μg   <0.1 eu/μg  <0.01 eu/μg  Not required
Monomer Isolation   Dimer Isolation   Not required
>80% by SDS-PAGE   >90% by SDS-PAGE  >95% by SDS-PAGE  Others
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45-1 Ramsey Road, Shirley, NY 11967, USA
USA: 1-631-559-9269  1-631-448-7888
Europe: 44-207-048-3343
FAX: 1-631-938-8127
EMAIL: info@creative-biomart.com

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