GMP Recombinant Human IFNG Protein

Cat.No.: IFNG-103HG
Product Overview: Recombinant Human IFNG, Gln24-Gln166, was produced in E. coli using non-animal reagents in an animal-free facility under cGMP guidelines.
Source: E. coli
Species: Human
Form: Lyophilized from a 0.2 μm filtered solution in Sodium Succinate, Mannitol and Tween® 80.
Bio-activity: Measured in anti-viral assays using HeLa human cervical epithelial carcinoma cells infected with encephalomyocarditis (EMC) virus. Meager, A. (1987) in Lymphokines and Interferons, a Practical Approach. Clemens, M.J. et al. (eds): IRL Press. 129. The ED50 for this effect is 0.15-0.75 ng/mL.
The specific activity of recombinant human IFN-gamma is approximately 2 x 10^4 IU/μg, which is calibrated against human IFN-gamma Standard (NIBSC code: 87/586).
Molecular Mass: Predicted Molecular Mass: 16.9 kDa
SDS-PAGE: 17 kDa, reducing conditions
N-terminal Sequence Analysis: Met-Gln24-Asp-Pro-Tyr-Val-Lys-Glu-Ala-Glu
Endotoxin: <0.01 EU per 1 μg of the protein by the LAL method.
Purity: >97%, by SDS-PAGE with silver staining, under reducing
Storage: Avoid repeated freeze-thaw cycles.
12 months, -20 to -70 centigrade as supplied.
1 month, 2 to 8 centigrade under sterile conditions after reconstitution.
3 months, -20 to -70 centigrade under sterile conditions after reconstitution.
Reconstitution: Reconstitute at 200 μg/mL in sterile, deionized water.
Quality Statement: Our GMP proteins are produced according to relevant sections of the following documents: WHO TRS, No. 822, 1992 Annex 1, Good Manufacturing Practices for Biological Products; USP Chapter 1043, Ancillary Materials for Cell, Gene and Tissue-Engineered Products and USP Chapter 92, Growth Factors and Cytokines Used in Cell Therapy Manufacturing.
Shipping: The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature.
Gene Name: IFNG interferon, gamma [ Homo sapiens ]
Official Symbol: IFNG
Synonyms: IFNG; interferon, gamma; interferon gamma; IFN-gamma; immune interferon; IFG; IFI;
Gene ID: 3458
mRNA Refseq: NM_000619
Protein Refseq: NP_000610
MIM: 147570
UniProt ID: P01579

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Optional requirements on this protein    +Expand
C-fusion    N-fusion   Non-tagged
His    GST   Fc   Others
<1.0 eu/μg    <0.1 eu/μg   <0.01 eu/μg   Not required
Monomer Isolation    Dimer Isolation    Not required
>80% by SDS-PAGE    >90% by SDS-PAGE   >95% by SDS-PAGE   Others

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