|Product Overview:||Recombinant Human IFNG, Gln24-Gln166, was produced in E. coli using non-animal reagents in an animal-free facility under cGMP guidelines.|
|Form:||Lyophilized from a 0.2 μm filtered solution in Sodium Succinate, Mannitol and Tween® 80.|
|Bio-activity:||Measured in anti-viral assays using HeLa human cervical epithelial carcinoma cells infected with encephalomyocarditis (EMC) virus. Meager, A. (1987) in Lymphokines and Interferons, a Practical Approach. Clemens, M.J. et al. (eds): IRL Press. 129. The ED50 for this effect is 0.15-0.75 ng/mL.
The specific activity of recombinant human IFN-gamma is approximately 2 x 10^4 IU/μg, which is calibrated against human IFN-gamma Standard (NIBSC code: 87/586).
|Molecular Mass:||Predicted Molecular Mass: 16.9 kDa
SDS-PAGE: 17 kDa, reducing conditions
|N-terminal Sequence Analysis:||Met-Gln24-Asp-Pro-Tyr-Val-Lys-Glu-Ala-Glu|
|Endotoxin:||<0.01 EU per 1 μg of the protein by the LAL method.|
|Purity:||>97%, by SDS-PAGE with silver staining, under reducing|
|Storage:||Avoid repeated freeze-thaw cycles.
12 months, -20 to -70 centigrade as supplied.
1 month, 2 to 8 centigrade under sterile conditions after reconstitution.
3 months, -20 to -70 centigrade under sterile conditions after reconstitution.
|Reconstitution:||Reconstitute at 200 μg/mL in sterile, deionized water.|
|Quality Statement:||Our GMP proteins are produced according to relevant sections of the following documents: WHO TRS, No. 822, 1992 Annex 1, Good Manufacturing Practices for Biological Products; USP Chapter 1043, Ancillary Materials for Cell, Gene and Tissue-Engineered Products and USP Chapter 92, Growth Factors and Cytokines Used in Cell Therapy Manufacturing.|
|Shipping:||The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature.|
|Gene Name:||IFNG interferon, gamma [ Homo sapiens ]|
|Synonyms:||IFNG; interferon, gamma; interferon gamma; IFN-gamma; immune interferon; IFG; IFI;|
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