Active GMP Recombinant Human IL11 Protein

• Cat.No.: IL11-101HG
• Product Overview: Recombinant Human IL11, Pro22-Leu199, was produced in Sf 9 (baculovirus)-derived in an animal component free process under cGMP guidelines.

Specification

• Source: Insect cell
• Species: Human
• Form: Lyophilized from a 0.2 μm filtered solution in PBS and EDTA.
• Bio-activity: Measured in a cell proliferation assay using T11 mouse plasmacytoma cells. Nordan, R.P. et al. (1987) J. Immunol. 139:813. The ED50 for this effect is 0.02-0.12 ng/mL.
  The specific activity of recombinant human IL-11 is approximately 1.05 x 10^4 units/μg, which is calibrated against recombinant human IL-11 WHO Standard (NIBSC code: 92/788).
• Molecular Mass: Predicted Molecular Mass: 19 kDa
  SDS-PAGE: 20 kDa, reducing conditions
• N-terminal Sequence Analysis: Pro-Gly-Pro-Pro-Pro-Gly-Pro-Pro-Arg-Val
• Endotoxin: <0.10 EU per 1 μg of the protein by the LAL method.
• Purity: >95%, by SDS-PAGE with silver staining, under reducing conditions.
• Storage: Avoid repeated freeze-thaw cycles.
  12 months, -20 to -70 centigrade as supplied.
  1 month, 2 to 8 centigrade under sterile conditions after reconstitution.
  3 months, -20 to -70 centigrade under sterile conditions after reconstitution.
• Reconstitution: Reconstitute at 100 μg/mL in PBS.
• Quality Statement: Our GMP proteins are produced according to relevant sections of the following documents: WHO TRS, No. 822, 1992 Annex 1, Good Manufacturing Practices for Biological Products; USP Chapter 1043, Ancillary Materials for Cell, Gene and Tissue-Engineered Products and USP Chapter 92, Growth Factors and Cytokines Used in Cell Therapy Manufacturing.
• Shipping: The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature.

Gene Information

• Gene Name: IL11 interleukin 11 [ Homo sapiens ]
• Official Symbol: IL11
• Synonyms: IL11; interleukin 11; interleukin-11; adipogenesis inhibitory factor; AGIF; IL 11; oprelvekin; IL-11
• Gene ID: 3589
• mRNA Refseq: NM_000641
• Protein Refseq: NP_000632
• MIM: 147681
• UniProt ID: P20809