Recombinant Human IL12B(Ile23–Ser328) and Human IL12A(Arg23–Ser219), was co-produced in Sf 21 (baculovirus)-derived under cGMP guidelines.
Lyophilized from a 0.2 μm filtered solution in PBS.
Measured in a cell proliferation assay using PHA-stimulated human T lymphoblasts. Symons, J.A. et al. (1987) in Lymphokines and Interferons, a Practical Approach. Clemens, M.J. et al. (eds): IRL Press. 272.
Predicted Molecular Mass: 34.7 (p40) & 22.5 (p35) kDa
SDS-PAGE: 41 kDa (p40) and 29 kDa (p35), reducing conditions
N-terminal Sequence Analysis:
<1.0 EU per 1 μg of the protein by the LAL method.
>97%, by SDS-PAGE with silver staining.
Avoid repeated freeze-thaw cycles.
12 months, -20 to -70 centigrade as supplied.
1 month, 2 to 8 centigrade under sterile conditions after reconstitution.
3 months, -20 to -70 centigrade under sterile conditions after reconstitution.
Reconstitute at 100 μg/mL in PBS.
Our GMP proteins are produced according to relevant sections of the following documents: WHO TRS, No. 822, 1992 Annex 1, Good Manufacturing Practices for Biological Products; USP Chapter 1043, Ancillary Materials for Cell, Gene and Tissue-Engineered Products and USP Chapter 92, Growth Factors and Cytokines Used in Cell Therapy Manufacturing.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature.