|Product Overview:||Recombinant Human IL2, Ala21-Thr153, was produced in E. coli using non-animal reagents in an animal-free facility under cGMP guidelines.|
|Form:||Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA.|
|Bio-activity:||Measured in a cell proliferation assay using CTLL‑2 mouse cytotoxic T cells. Gearing, A.J.H. and C.B. Bird (1987) in Lymphokines and Interferons, A Practical Approach. Clemens, M.J. et al. (eds): IRL Press. 295.|
|Molecular Mass:||Predicted Molecular Mass: 15.5 kDa
SDS-PAGE: 13 kDa, reducing conditions
|N-terminal Sequence Analysis:||Met-Ala21-Pro-Thr-Ser-Ser-Ser-Thr-Lys-Lys|
|Endotoxin:||<0.01 EU per 1 μg of the protein by the LAL method.|
|Purity:||>97%, by SDS-PAGE with silver staining, under reducing conditions. Intact mass analysis of recombinant human IL-2 confirms the predicted molecular mass of 15549 Da.|
|Storage:||Avoid repeated freeze-thaw cycles.
12 months, -20 to -70 centigrade as supplied.
1 month, 2 to 8 centigrade under sterile conditions after reconstitution.
3 months, -20 to -70 centigrade under sterile conditions after reconstitution.
|Reconstitution:||Reconstitute at 100 μg/mL in 100 mM acetic acid|
|Quality Statement:||Our GMP proteins are produced according to relevant sections of the following documents: WHO TRS, No. 822, 1992 Annex 1, Good Manufacturing Practices for Biological Products; USP Chapter 1043, Ancillary Materials for Cell, Gene and Tissue-Engineered Products and USP Chapter 92, Growth Factors and Cytokines Used in Cell Therapy Manufacturing.|
|Shipping:||The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature.|
|Gene Name:||IL2 interleukin 2 [ Homo sapiens ]|
|Synonyms:||IL2; interleukin 2; interleukin-2; IL 2; T cell growth factor; TCGF; aldesleukin; IL-2; lymphokine;|
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