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GMP Recombinant Human IL2 protein

Cat.No. : IL2-4317HG
Product Overview : Recombinant Human IL2 protein was expressed in Escherichia coli. This product is produced under cGMP guidelines.
  • Specification
  • Gene Information
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Description : IL-2 is a powerful immunoregulatory lymphokine produced by T-cells in response to antigenic or mitogenic stimulation. It is expressed by CD4+ and CD8+ T cells, γδ T cells, B cells, dendritic cells, and eosinophils. IL-2/IL-2R signaling is required for T-cell proliferation and other fundamental functions which are essential for the immune response. The receptor for IL-2 consists of three subunits (55 kDa IL2Rα, 75 kDa IL2Rβ, 64 kDa common gamma chain γc/IL2Rγ) that are present on the cell surface in varying preformed complexes. Mature human IL-2 shares 56 % and 66 % amino acid sequence identity with mouse and rat IL-2, respectively. Human and mouse IL-2 exhibit cross-species activity.
Source : E.coli
Species : Human
Form : Lyophilized from a 0.2μm filtered concentrated solution in 20 mM PB, pH 3.5.
Bio-activity : Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using murine CTLL-2 cells is less than 0.1 ng/ml, corresponding to a specific activity of > 1.0 × 10⁷ IU/mg.
Molecular Mass : Approximately 15.4 kDa, a single non-glycosylated polypeptide chain containing 133 amino acids.
Protein length : 133
AA Sequence : APTSSSTKKTQLQLEHLLLDLQMIL NGINNYKNPKLTRMLTFKFYMPKKA TELKHLQCLEEELKPLEEVLNLAQS KNFHLRPRDLISNINVIVLELKGSE TTFMCEYADETATIVEFLNRWITFC QSIISTLT
Endotoxin : Less than 0.01 EU/µg of rHuIL-2 GMP as determined by LAL method.
Purity : >98% by SDS-PAGE and HPLC analysis.
Storage : Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 centigrade as supplied. 1 month, 2 to 8 centigrade under sterile conditions after reconstitution. 3 months, -20 to -70 centigrade under sterile conditions after reconstitution.
Reconstitution : We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤-20 centigrade. Further dilutions should be made in appropriate buffered solutions. Do not reconstitute in cell culture media directly.
Gene Name : IL2
Official Symbol : IL2
Synonyms : IL2; interleukin 2; interleukin-2; IL 2; T cell growth factor; TCGF; aldesleukin; involved in regulation of T-cell clonal expansion; IL-2; lymphokine;
Gene ID : 3558
mRNA Refseq : NM_000586
Protein Refseq : NP_000577
MIM : 147680
UniProt ID : P60568

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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Q&As (10)

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What is the impact of IL2 on T-cell memory formation? 04/25/2022

IL2 promotes the formation of memory T-cells.

What is the role of IL2 in the tumor microenvironment? 12/12/2021

IL2 has a pro-tumorigenic role in the tumor microenvironment.

How does IL2 interact with other cytokines in a mixed environment? 11/22/2021

IL2 synergizes with IL-12 in a mixed cytokine environment.

Does IL2 modulate the expression of checkpoint inhibitors? 06/03/2020

IL2 upregulates the expression of PD-1 checkpoint inhibitors.

What are the kinetics of IL2 internalization in T cells? 08/18/2019

IL2 is rapidly internalized by activated T-cells.

Does IL2 influence the polarization of macrophages? 07/24/2019

IL2 induces M1 macrophage polarization.

How does IL2 affect the blood-brain barrier permeability? 07/06/2018

IL2 increases blood-brain barrier permeability.

How does IL2's affinity for its receptor change under hypoxic conditions? 04/11/2018

IL2's affinity for its receptor decreases under hypoxic conditions.

What post-translational modifications affect IL2's bioactivity? 02/19/2017

IL2's bioactivity is enhanced by glycosylation.

What is the role of IL2 in the differentiation of Th17 cells? 04/20/2016

IL2 promotes the differentiation of Th17 cells.

Customer Reviews (3)

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Reviews
04/13/2022

    Potent in T-cell activation.

    07/27/2018

      Suitable for cytokine assays.

      01/07/2018

        High stability.

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