Recombinant Human IL7, Asp26-His177, was produced in E. coli using non-animal reagents in an animal-free facility under cGMP guidelines.
Lyophilized from a 0.2 μm filtered solution in PBS.
Measured in a cell proliferation assay using PHA-activated human peripheral blood lymphocytes (PBL). Yokota, T. et al. (1986) Proc. Natl. Acad. Sci. USA 83:5894. The ED50 for this effect is 0.1-0.5 ng/mL.
The specific activity of Recombinant Human IL-7 is approximately 4.4 x 10^5 units/μg, which is calibrated against human IL-7 WHO Standard (NIBSC code: 90/530).
Predicted Molecular Mass: 17 kDa
N-terminal Sequence Analysis:
<0.10 EU per 1 μg of the protein by the LAL method.
>97%, by SDS-PAGE with silver staining, under reducing conditions.
Avoid repeated freeze-thaw cycles.
12 months, -20 to -70 centigrade as supplied.
1 month, 2 to 8 centigrade under sterile conditions after reconstitution.
3 months, -20 to -70 centigrade under sterile conditions after reconstitution.
Reconstitute at 100 μg/mL in PBS.
Our GMP proteins are produced according to relevant sections of the following documents: WHO TRS, No. 822, 1992 Annex 1, Good Manufacturing Practices for Biological Products; USP Chapter 1043, Ancillary Materials for Cell, Gene and Tissue-Engineered Products and USP Chapter 92, Growth Factors and Cytokines Used in Cell Therapy Manufacturing.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature.