Recombinant Human MSTN, Asp268-Ser376, was produced in NS0 cell under cGMP guidelines.
Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA.
Measured by its ability to induce hemoglobin expression in K562 human chronic myelogenous leukemia cells. Schwall, R.H. et al. (1991) Method Enzymol. 198:340.
Predicted Molecular Mass: 12.4 kDa
SDS-PAGE: 12 kDa, reducing conditions; 24 kDa, non-reducing conditions
N-terminal Sequence Analysis:
<0.10 EU per 1 μg of the protein by the LAL method.
>90%, by SDS-PAGE with silver staining.
Avoid repeated freeze-thaw cycles.
12 months, -20 to -70 centigrade as supplied.
1 month, 2 to 8 centigrade under sterile conditions after reconstitution.
3 months, -20 to -70 centigrade under sterile conditions after reconstitution.
Reconstitute at 100 μg/mL in 4 mM HCl.
Our GMP proteins are produced according to relevant sections of the following documents: WHO TRS, No. 822, 1992 Annex 1, Good Manufacturing Practices for Biological Products; USP Chapter 1043, Ancillary Materials for Cell, Gene and Tissue-Engineered Products and USP Chapter 92, Growth Factors and Cytokines Used in Cell Therapy Manufacturing.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature.