|Product Overview:||Recombinant Human PDGFB, Ser82-Thr190, was produced in E. coli using non-animal reagents in an animal-free facility under cGMP guidelines.|
|Form:||Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA.|
|Bio-activity:||Measured in a cell proliferation assay using NR6R‑3T3 mouse fibroblast cells. Raines, E.W. et al. (1985) Methods Enzymol. 109:749.|
|Molecular Mass:||Predicted Molecular Mass: 12.3 (monomer) kDa|
|N-terminal Sequence Analysis:||Ser-Leu-Gly-Ser-Leu-Thr-Ile-Ala-Glu-Pro|
|Endotoxin:||<0.01 EU per 1 μg of the protein by the LAL method.|
|Purity:||>97%, by SDS-PAGE with silver staining, under reducing conditions.|
|Storage:||Avoid repeated freeze-thaw cycles.
12 months, -20 to -70 centigrade as supplied.
1 month, 2 to 8 centigrade under sterile conditions after reconstitution.
3 months, -20 to -70 centigrade under sterile conditions after reconstitution.
|Reconstitution:||Reconstitute at 100 μg/mL in 4 mM HCl.|
|Quality Statement:||Our GMP proteins are produced according to relevant sections of the following documents: WHO TRS, No. 822, 1992 Annex 1, Good Manufacturing Practices for Biological Products; USP Chapter 1043, Ancillary Materials for Cell, Gene and Tissue-Engineered Products and USP Chapter 92, Growth Factors and Cytokines Used in Cell Therapy Manufacturing.|
|Shipping:||The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature.|
|Gene Name:||PDGFB platelet-derived growth factor beta polypeptide [ Homo sapiens ]|
|Synonyms:||PDGFB; platelet-derived growth factor beta polypeptide; becaplermin; oncogene SIS; SSV; PDGF-2; PDGF, B chain; PDGF subunit B; proto-oncogene c-Sis; platelet-derived growth factor 2; SIS; PDGF2; c-sis|
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