Active GMP Recombinant Human PDGFB Protein

• Cat.No.: PDGFB-125HG
• Product Overview: Recombinant Human PDGFB, Ser82-Thr190, was produced in E. coli using non-animal reagents in an animal-free facility under cGMP guidelines.

Specification

• Source: E. coli
• Species: Human
• Form: Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA.
• Bio-activity: Measured in a cell proliferation assay using NR6R‑3T3 mouse fibroblast cells. Raines, E.W. et al. (1985) Methods Enzymol. 109:749.
• Molecular Mass: Predicted Molecular Mass: 12.3 (monomer) kDa
• N-terminal Sequence Analysis: Ser-Leu-Gly-Ser-Leu-Thr-Ile-Ala-Glu-Pro
• Endotoxin: <0.01 EU per 1 μg of the protein by the LAL method.
• Purity: >97%, by SDS-PAGE with silver staining, under reducing conditions.
• Storage: Avoid repeated freeze-thaw cycles.
  12 months, -20 to -70 centigrade as supplied.
  1 month, 2 to 8 centigrade under sterile conditions after reconstitution.
  3 months, -20 to -70 centigrade under sterile conditions after reconstitution.
• Reconstitution: Reconstitute at 100 μg/mL in 4 mM HCl.
• Quality Statement: Our GMP proteins are produced according to relevant sections of the following documents: WHO TRS, No. 822, 1992 Annex 1, Good Manufacturing Practices for Biological Products; USP Chapter 1043, Ancillary Materials for Cell, Gene and Tissue-Engineered Products and USP Chapter 92, Growth Factors and Cytokines Used in Cell Therapy Manufacturing.
• Shipping: The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature.

Gene Information

• Gene Name: PDGFB platelet-derived growth factor beta polypeptide [ Homo sapiens ]
• Official Symbol: PDGFB
• Synonyms: PDGFB; platelet-derived growth factor beta polypeptide; becaplermin; oncogene SIS; SSV; PDGF-2; PDGF, B chain; PDGF subunit B; proto-oncogene c-Sis; platelet-derived growth factor 2; SIS; PDGF2; c-sis
• Gene ID: 5155
• mRNA Refseq: NM_002608
• Protein Refseq: NP_002599
• MIM: 190040
• UniProt ID: P01127