Active GMP Recombinant Human TGFB1 Protein

• Cat.No.: TGFB1-134HG
• Product Overview: Recombinant Human TGFB1, Ala279-Ser390, was produced in CHO cell under cGMP guidelines.

Specification

• Source: CHO cell
• Species: Human
• Form: Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA.
• Bio-activity: Measured by its ability to inhibit the IL-4-dependent proliferation of HT‑2 mouse T cells. Tsang, M. et al. (1995) Cytokine 7:389. The specific activity of recombinant human TGF-beta 1 GMP is approximately 2.5 x 10^4 U/μg, which is calibrated against human TGF‑ beta 1 Standard (NIBSC code: 89/514).
• Molecular Mass: Predicted Molecular Mass: 12.8 (monomer) kDa
  SDS-PAGE: 12 kDa, reducing conditions; 24 kDa, non-reducing conditions
• N-terminal Sequence Analysis: Ala-Leu-Asp-Thr-Asn-Tyr-(Cys)-Phe-Ser-Ser
• Endotoxin: <0.10 EU per 1 μg of the protein by the LAL method.
• Purity: >97%, by SDS-PAGE with silver staining.
• Storage: Avoid repeated freeze-thaw cycles.
  12 months, -20 to -70 centigrade as supplied.
  1 month, 2 to 8 centigrade under sterile conditions after reconstitution.
  3 months, -20 to -70 centigrade under sterile conditions after reconstitution.
• Reconstitution: Reconstitute at 100 μg/mL in 4 mM HCl.
• Quality Statement: Our GMP proteins are produced according to relevant sections of the following documents: WHO TRS, No. 822, 1992 Annex 1, Good Manufacturing Practices for Biological Products; USP Chapter 1043, Ancillary Materials for Cell, Gene and Tissue-Engineered Products and USP Chapter 92, Growth Factors and Cytokines Used in Cell Therapy Manufacturing.
• Shipping: The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature.

Gene Information

• Gene Name: TGFB1 transforming growth factor, beta 1 [ Homo sapiens ]
• Official Symbol: TGFB1
• Synonyms: TGFB1; transforming growth factor, beta 1; DPD1, TGFB; transforming growth factor beta-1; Camurati Engelmann disease; CED; TGFbeta; TGF-beta-1; TGF-beta 1 protein; LAP; DPD1; TGFB
• Gene ID: 7040
• mRNA Refseq: NM_000660
• Protein Refseq: NP_000651
• MIM: 190180
• UniProt ID: P01137