Address: 45-1 Ramsey Road, Shirley, NY 11967, USA    USA: 1-631-559-9269  1-631-448-7888   Fax: 1-631-938-8127  Europe: 44-207-097-1828   Email: info@creative-biomart.com
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GMP Recombinant Human TGFB3 Protein

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Cat.No.:
TGFB3-104HG
Product Overview:
Recombinant Human TGFB3, Ala301-Ser412, was produced in Sf 9 (baculovirus)-derived in an animal component free process under cGMP guidelines.
Source:
Insect cell
Species:
Human
Form:
Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA.
Bio-activity:
Measured by its ability to inhibit the IL-4-dependent proliferation of HT‑2 mouse T cells. Tsang, M. et al. (1995) Cytokine 7:389. The ED50 for this effect is 0.01-0.04 ng/mL.
The specific activity of recombinant human TGF-beta 3 is approximately 2.2 x 10^4 IU/μg, which is calibrated against recombinant human TGF-beta 3 WHO International Standard (NIBSC code: 09/234).
Molecular Mass:
Predicted Molecular Mass: 12.7 (monomer) kDa
SDS-PAGE: 12 kDa, reducing conditions; 24 kDa, non-reducing conditions
N-terminal Sequence Analysis:
Ala301-Leu-Asp-Thr-Asn-Tyr-(Cys)-Phe-Arg-Asn
Endotoxin:
<0.01 EU per 1 μg of the protein by the LAL method.
Purity:
>97%, by SDS-PAGE with silver staining.
Storage:
Avoid repeated freeze-thaw cycles.
12 months, -20 to -70 centigrade as supplied.
1 month, 2 to 8 centigrade under sterile conditions after reconstitution.
3 months, -20 to -70 centigrade under sterile conditions after reconstitution.
Reconstitution:
Reconstitute at 50-200 μg/mL in sterile 4 mM HCl.
Quality Statement:
Our GMP proteins are produced according to relevant sections of the following documents: WHO TRS, No. 822, 1992 Annex 1, Good Manufacturing Practices for Biological Products; USP Chapter 1043, Ancillary Materials for Cell, Gene and Tissue-Engineered Products and USP Chapter 92, Growth Factors and Cytokines Used in Cell Therapy Manufacturing.
Shipping:
The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature.


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Note: There will be extra charge for optional service!
Optional requirements on this protein +Expand
C-fusion   N-fusion  Non-tagged
His   GST  Fc  Others
<1.0 eu/μg   <0.1 eu/μg  <0.01 eu/μg  Not required
Monomer Isolation   Dimer Isolation   Not required
>80% by SDS-PAGE   >90% by SDS-PAGE  >95% by SDS-PAGE  Others
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45-1 Ramsey Road, Shirley, NY 11967, USA
USA: 1-631-559-9269  1-631-448-7888
Europe: 44-207-097-1828
FAX: 1-631-938-8127
EMAIL: info@creative-biomart.com

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