GPD Activity Assay Kit (Colorimetric) is used for measuring glycerol-3-phosphate Dehydrogenase activity using colorimetric methods.
Glycerol-3-Phosphate Dehydrogenase (EC 220.127.116.11) is an important enzyme for lipid metabolism. It catalyzes the reversible conversion between dihydroxyacetone phosphate and glycerol-3-phosphate. GPDH plays multiple functions inside cells; it links carbohydrate and lipid metabolism, and provides electrons through the Glycerol-3-Phosphate Shuttle. When progenitor adipocytes differentiate into mature adipocytes, GPDH activity increases significantly. Analysis of glycerol-3-phosphate dehydrogenase activity is crucial for the study of fatty acid metabolic pathways. In GPD Activity Assay Kit (Colorimetric), Glycerol-3-phosphate dehydrogenase reacts with the substrate to forms an intermediate, which reduces a colorless probe to a colored product with strong absorbance at 450 nm. The assay is simple, sensitive and rapid and can detect Glycerol-3-Phosphate dehydrogenase activity less than 1 mU/well.
Measurement of Glycerol-3-Phosphate Dehydrogenase activity in various tissues/cellsAnalysis of fatty acid metabolism and cell signalingScreening anti-obesity drugs
For research use only (RUO)
Store kit at –20°C, protected from light. Warm GPDH Assay Buffer to room temperature before use. Briefly centrifuge small vials prior to opening.
GPDH Assay Buffer 27 mlGPDH Substrate (Lyophilized) 1 vialGPDH Probe (Lyophilized) 1 vialNADH Standard (Lyophilized) 1 vialGPDH Positive Control (Lyophilized) 1 vial
Compatible Sample Types:
Cell culture, Tissue samples