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Home > Products > Assay Kits > Activity Kits > GPD Activity Colorimetric Assay Kit

GPD Activity Colorimetric Assay Kit

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Product Overview:
GPD Activity Assay Kit (Colorimetric) is used for measuring glycerol-3-phosphate Dehydrogenase activity using colorimetric methods.
Glycerol-3-Phosphate Dehydrogenase (EC is an important enzyme for lipid metabolism. It catalyzes the reversible conversion between dihydroxyacetone phosphate and glycerol-3-phosphate. GPDH plays multiple functions inside cells; it links carbohydrate and lipid metabolism, and provides electrons through the Glycerol-3-Phosphate Shuttle. When progenitor adipocytes differentiate into mature adipocytes, GPDH activity increases significantly. Analysis of glycerol-3-phosphate dehydrogenase activity is crucial for the study of fatty acid metabolic pathways. In GPD Activity Assay Kit (Colorimetric), Glycerol-3-phosphate dehydrogenase reacts with the substrate to forms an intermediate, which reduces a colorless probe to a colored product with strong absorbance at 450 nm. The assay is simple, sensitive and rapid and can detect Glycerol-3-Phosphate dehydrogenase activity less than 1 mU/well.
Measurement of Glycerol-3-Phosphate Dehydrogenase activity in various tissues/cellsAnalysis of fatty acid metabolism and cell signalingScreening anti-obesity drugs
For research use only (RUO)
Store kit at –20°C, protected from light. Warm GPDH Assay Buffer to room temperature before use. Briefly centrifuge small vials prior to opening.
Kit Components:
GPDH Assay Buffer 27 mlGPDH Substrate (Lyophilized) 1 vialGPDH Probe (Lyophilized) 1 vialNADH Standard (Lyophilized) 1 vialGPDH Positive Control (Lyophilized) 1 vial
Detection method:
Compatible Sample Types:
Cell culture, Tissue samples

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Optional requirements on this protein +Expand
C-fusion   N-fusion  Non-tagged
His   GST  Fc  Others
<1.0 eu/μg   <0.1 eu/μg  <0.01 eu/μg  Not required
Monomer Isolation   Dimer Isolation   Not required
>80% by SDS-PAGE   >90% by SDS-PAGE  >95% by SDS-PAGE  Others
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45-1 Ramsey Road, Shirley, NY 11967, USA
USA: 1-631-559-9269  1-631-448-7888
Europe: 44-207-048-3343
FAX: 1-631-938-8127

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