"GZMB" Related Products

Expand
Expand
Expand

GZMB Activity Fluorometric Assay Kit

Cat.No.: Kit-0403
Product Overview: GZMB Activity Assay Kit (Fluorometric) is used for measuring granzyme B activity using fluorometric methods.
Description: Granzyme B (GZMB, EC number 3.4.21.79) also known as Granzyme-2 is a serine protease stored in the granules of activated cytotoxic T cells and NK cells. Upon target cell contact, Granzyme B is directionally exocytosed and with the assistance of perforin enters the target cell. With its unique substrate specificity (Granzyme B prefers an aspartic acid residue at the P1 site of its substrates), Granzyme B processes and activates various pro-caspases thereby inducing apoptosis in the target cell. In GZMB Activity Assay Kit (Fluorometric), we have utilized a peptide substrate containing the Granzyme B recognition sequence along with a fluorescent label "AFC". Granzyme B catalyzes the cleavage of this substrate and releases the AFC molecule, which can be detected fluorometrically (Ex/Em = 380/500 nm). This Assay Kit is rapid, reliable and sensitive and can detect Granzyme B activity in various biological samples.
Applications: Measurement of Granzyme B activity in various biological samples or purified Granzyme B activity.Study & characterize Granzyme B inhibitors.
Usage: For research use only (RUO)
Storage: Store kit at -20°C, protected from light. Warm Assay Buffer to room temperature before use. Briefly centrifuge small vials prior to opening.
Kit Components: Granzyme B Assay Buffer 25 mlGranzyme B Substrate 0.5 mlPositive control (Granzyme B Enzyme, human recombinant) 1 vialAFC Standard (1 mM) 0.1 ml
Detection method: Fluorometric
Compatible Sample Types: Biological samples

Online Inquiry

Note: There will be extra charge for optional service!
Optional requirements on this protein    +Expand
C-fusion    N-fusion   Non-tagged
His    GST   Fc   Others
<1.0 eu/μg    <0.1 eu/μg   <0.01 eu/μg   Not required
Monomer Isolation    Dimer Isolation    Not required
>80% by SDS-PAGE    >90% by SDS-PAGE   >95% by SDS-PAGE   Others

Please input "biomart" as verification code. Please review Creative BioMart's privacy policy for more information


Price does not include shipping and packaging costs.Request quote for final price.

Price Inquiry

Welcome! For price inquiries, please feel free to contact us through the form below. We will get back to you as soon as possible.