GZMB Assay/Inhibitor Screening Kit (Fluorometric) is used for screening granzyme B inhibitors or characterize/study of granzyme B inhibitors using fluorometric methods.
Granzyme B (GZMB, EC number 220.127.116.11) also known as Granzyme-2 is a serine protease stored in the granules of activated cytotoxic T cells and NK cells. Upon target cell contact, Granzyme B is directionally exocytosed and with the assistance of perforin enters the target cell. With its unique substrate specificity (Granzyme B prefers an aspartic acid residue at the P1 site of its substrates), Granzyme B processes and activates various pro-caspases thereby inducing apoptosis in the target cell. In GZMB Activity Assay Kit (Fluorometric), we have utilized a peptide substrate containing the Granzyme B recognition sequence along with a fluorescent label "AFC". Granzyme B catalyzes the cleavage of this substrate and releases the AFC molecule, which can be detected fluorometrically (Ex/Em = 380/500 nm). This Assay Kit is rapid, reliable and sensitive and can detect Granzyme B activity in various biological samples.
Screening Granzyme B inhibitors.Characterize/Study Granzyme B inhibitors.
For research use only (RUO)
Store kit at -20°C, protected from light. Warm Assay Buffer to room temperature before use. Briefly centrifuge small vials before opening. Read the entire protocol before performing the assay.
Granzyme B Assay Buffer 25 mlGranzyme B Substrate 500 μlGranzyme B Enzyme (human recombinant) 1 vialInhibitor Control (Ac-IEPD-CHO, 250 μM) 20 μl