Address: 45-1 Ramsey Road, Shirley, NY 11967, USA    USA: 1-631-559-9269  1-631-448-7888   Fax: 1-631-938-8127  Europe: 44-207-048-3343   Email: info@creative-biomart.com
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Galactose and Lactose Assay Kit

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Cat.No.:
Kit-0356
Product Overview:
Galactose and Lactose Assay Kit is used for measuring of galactose and lactose levels.
Description:
Galactose (C6H12O6 FW: 180.16) and lactose (C12H22O11 FW: 342.3) are naturally occurring sugars. Lactose consists of one galactose and one glucose. Some people, particularly infants, lack the enzyme necessary to digest galactose leading to galactose accumulation in blood (Galactosemia) causing enlarged liver, renal failure, cataracts and brain damage. Galactose and Lactose Assay Kit provides a simple, convenient means for direct measurement of galactose and lactose levels in various biological samples (serum, plasma, other body fluids, food, growth media, etc.). To detect Galactose, galactose oxidase specifically oxidizes free galactose, generating a product that reacts with the Galactose Probe to produce color (O.D 570 nm) and fluorescence (Ex/Em 535/587). To detect lactose, lactose is hydrolyzed by lactase to generate free galactose which is then measured. Thus, Lactose level = Total Galactose - Free Galactose.
Target Species:
Mammals
Usage:
For research use only (RUO)
Storage:
Store kit at -20°C, protect from light. Allow reagents warm to room temperature and briefly centrifuge vials before opening.
Kit Components:
Galactose Assay Buffer 25 mLGalactose Probe* (DMSO solution) 0.2 mLLactase (Lyophilized) 1 vialGalactose Enzyme Mix (Lyophilized) 1 vialHRP (Lyophilized) 1 vialGalactose Standard (100 nmol/μL) 100 μL
Detection method:
Colorimetric, Fluorometric
Compatible Sample Types:
Biological Fluid, Cell Culture Supernatant, Plasma, Serum, Tissue Culture Supernatant, Urine


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Note: There will be extra charge for optional service!
Optional requirements on this protein +Expand
C-fusion   N-fusion  Non-tagged
His   GST  Fc  Others
<1.0 eu/μg   <0.1 eu/μg  <0.01 eu/μg  Not required
Monomer Isolation   Dimer Isolation   Not required
>80% by SDS-PAGE   >90% by SDS-PAGE  >95% by SDS-PAGE  Others
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45-1 Ramsey Road, Shirley, NY 11967, USA
USA: 1-631-559-9269  1-631-448-7888
Europe: 44-207-048-3343
FAX: 1-631-938-8127
EMAIL: info@creative-biomart.com

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