Address: 45-1 Ramsey Road, Shirley, NY 11967, USA    USA: 1-631-559-9269  1-631-448-7888   Fax: 1-631-938-8127  Europe: 44-207-048-3343   Email: info@creative-biomart.com
Home > Products > Assay Kits > Activity Kits > Glucose Oxidase Activity Assay Kit

Glucose Oxidase Activity Assay Kit

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Cat.No.:
Kit-0383
Product Overview:
Glucose Oxidase Activity Assay Kit is a quantitative colormetric/fluorometric determination of glucose oxidase activity.
Description:
The glucose oxidase enzyme (GOx) (EC 1.1.3.4) is an oxidoreductase commonly found in a wide variety of microorganisms that catalyzes the oxidation of glucose to hydrogen peroxide and D-glucono-δ-lactone. GOx aids in breaking the sugar down into its metabolites. Glucose Oxidase Activity Assay Kit provides a convenient tool for sensitive detection of the GOx in a variety of samples. Glucose oxidase in samples recognizes Dglucose as a specific substrate leading to proportional color development. The activity of GOx can be easily quantified colorimetrically (λ = 570 nm) or fluorometrically (Ex/Em = 535/585 nm). GOx assay detects glucose oxidase activity as low as 0.01mU.
Usage:
For research use only (RUO)
Storage:
Store kit at -20°C, protected from light. Warm Assay Buffer to room temperature before use. Briefly centrifuge all small vials prior to opening. Read the entire protocol before performing the assay.
Kit Components:
GOx Assay Buffer. Cap code: WM. 28 mlOxiRed Probe. Cap code: red. 0.2 mlGOx Substrate. Cap code: blue. 1 mlGOx Developer. Cap code: green. 1 vialGOx Positive Control. Cap code: purple. 1 vialH2O2 Standard (0.88 M). Cap code: yellow. 0.1 ml
Detection method:
Colorimetric, Fluorometric
Compatible Sample Types:
Biological Fluid, Cell Homogenate


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Optional requirements on this protein +Expand
C-fusion   N-fusion  Non-tagged
His   GST  Fc  Others
<1.0 eu/μg   <0.1 eu/μg  <0.01 eu/μg  Not required
Monomer Isolation   Dimer Isolation   Not required
>80% by SDS-PAGE   >90% by SDS-PAGE  >95% by SDS-PAGE  Others
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45-1 Ramsey Road, Shirley, NY 11967, USA
USA: 1-631-559-9269  1-631-448-7888
Europe: 44-207-048-3343
FAX: 1-631-938-8127
EMAIL: info@creative-biomart.com

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