Glucose Oxidase Activity Assay Kit is a quantitative colormetric/fluorometric determination of glucose oxidase activity.
The glucose oxidase enzyme (GOx) (EC 220.127.116.11) is an oxidoreductase commonly found in a wide variety of microorganisms that catalyzes the oxidation of glucose to hydrogen peroxide and D-glucono-δ-lactone. GOx aids in breaking the sugar down into its metabolites. Glucose Oxidase Activity Assay Kit provides a convenient tool for sensitive detection of the GOx in a variety of samples. Glucose oxidase in samples recognizes Dglucose as a specific substrate leading to proportional color development. The activity of GOx can be easily quantified colorimetrically (λ = 570 nm) or fluorometrically (Ex/Em = 535/585 nm). GOx assay detects glucose oxidase activity as low as 0.01mU.
For research use only (RUO)
Store kit at -20°C, protected from light. Warm Assay Buffer to room temperature before use. Briefly centrifuge all small vials prior to opening. Read the entire protocol before performing the assay.
GOx Assay Buffer. Cap code: WM. 28 mlOxiRed Probe. Cap code: red. 0.2 mlGOx Substrate. Cap code: blue. 1 mlGOx Developer. Cap code: green. 1 vialGOx Positive Control. Cap code: purple. 1 vialH2O2 Standard (0.88 M). Cap code: yellow. 0.1 ml
Compatible Sample Types:
Biological Fluid, Cell Homogenate