Glucose Oxidase Assay Kit (Fluorometric) provides a quick and sensitive method for the measurement of glucose oxidase in solution. It can be performed in a convenient 96-well or 384-well microtiter plate format and readily adapted to automation without a separation step. The kit uses our AbRed Indicator which enables a dual recordable mode. The fluorescent signal can be easily read by either a fluorescence microplate reader at Ex/Em = 540/590 nm or an absorbance microplate reader at 576 nm.
Glucose oxidase is a dimeric protein that catalyzes the oxidation of beta-D-glucose into hydrogen peroxide and D-glucono-1,5-lactone, which is hydrolyzed to gluconic acid. It is widely used for the determination of glucose in body fluids and in removing residual glucose and oxygen from beverages, food and other agricultural products. Furthermore, Glucose oxidase is commonly used in biosensors to detect glucose.
Reacts with: HumanPredicted to work with: all Mammals
Keep at -20°C. Avoid exposure to light
AbRed Indicator 1 vialAssay Buffer 1 x 50mlDMSO 1 x 200µlGlucose oxidase 1 vialGlucose standard 1 vialHorseradish Peroxidase 1 vial
Compatible Sample Types:
Urine, Plasma, Other biological fluids