Address: 45-1 Ramsey Road, Shirley, NY 11967, USA    USA: 1-631-559-9269  1-631-448-7888   Fax: 1-631-938-8127  Europe: 44-207-048-3343   Email: info@creative-biomart.com
Home > Products > Assay Kits > Activity Kits > Glutamate Dehydrogenase Activity Assay Kit

Glutamate Dehydrogenase Activity Assay Kit

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Cat.No.:
Kit-0358
Product Overview:
GDH Activity Assay Kit is used for measuring glucose dehydrogenase activity.
Description:
Glucose 1-dehydrogenase (NAD+) (EC 1.1.1.118) is an enzyme that catalyzes the chemical reaction: D-glucose + NAD+ ↔ D-glucono-1,5-lactone + NADH + H+. This enzyme belongs to the family of oxidoreductases, specifically those acting on the CH-OH group of donor with NAD+ or NADP+ as acceptor. GDH Activity Assay Kit provides a convenient tool for sensitive detection of the GDH in a variety of samples. The GDH present in sample will recognize D-glucose as a specific substrate leading to a proportional color development. The activity of GDH can be easily quantified colorimetrically (λ = 450 nm). This assay detects GDH activity as low as 0.01 mU with our unit definition.
Target Species:
Mammals
Usage:
For research use only (RUO)
Storage:
Store the kit at -20°C, protect from light. Allow Assay Buffer to warm to room temperature before use. Briefly centrifuge vials prior to opening. Read the entire protocol before performing the assay.
Kit Components:
GDH Assay Buffer. Cap code: WM. 25 mLGlucose (2 M). Cap code: blue. 1 mLDeveloper (lyophilized). Cap code: red. 1 vialGDH Positive Control (lyophilized). Cap code: green. 1 vialNADH Standard (0.5 μmol, Lyophilized). Cap code: yellow. 1 vial
Detection method:
Colorimetric
Compatible Sample Types:
Biological Fluid, Cell Culture Supernatant, Culture Medium, Fermentation Medium, Food Sample, Plasma, Serum, Tissue Culture Supernatant, Urine


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Optional requirements on this protein +Expand
C-fusion   N-fusion  Non-tagged
His   GST  Fc  Others
<1.0 eu/μg   <0.1 eu/μg  <0.01 eu/μg  Not required
Monomer Isolation   Dimer Isolation   Not required
>80% by SDS-PAGE   >90% by SDS-PAGE  >95% by SDS-PAGE  Others
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45-1 Ramsey Road, Shirley, NY 11967, USA
USA: 1-631-559-9269  1-631-448-7888
Europe: 44-207-048-3343
FAX: 1-631-938-8127
EMAIL: info@creative-biomart.com

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