Address: 45-1 Ramsey Road, Shirley, NY 11967, USA    USA: 1-631-559-9269  1-631-448-7888   Fax: 1-631-938-8127  Europe: 44-207-048-3343   Email: info@creative-biomart.com
Home > Products > Assay Kits > Metabolism Kits > Glutamine Colorimetric Assay Kit

Glutamine Colorimetric Assay Kit

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Cat.No.:
Kit-0370
Product Overview:
Glutamine Assay Kit (Colorimetric) is used to determine glutamine.
Description:
Glutamine (Gln) is one of the most abundant amino acids containing an uncharged amide as a side chain. It is synthesized via condensation of glutamate and ammonia. Gln is classified as a non-essential amino acid, however, Gln is important in several biological processes such as protein synthesis, regulation of acid-balance in mammalian kidneys and cell growth. It constitutes cell"s main source of nitrogen for the synthesis of nucleotides and hexosamines. Glutamine-rich diets benefit patients suffering from Crohn"s disease, severe burns, HIV/AIDS and cancer. Glutamine Colorimetric Assay kit is a simple and sensitive assay that detects the biologically relevant concentrations of Gln in various fluids and tissues. The assay is based on the hydrolysis of Glutamine to Glutamate producing a stable signal, which is directly proportional to the amount of Gln in the sample. The assay can detect as little as 25 µM of Gln in a variety of biological samples.
Applications:
Measurement of Glutamine in various biological samples
Target Species:
Mammals
Usage:
For research use only (RUO)
Storage:
Store the kit at -20°C, protected from light.
Kit Components:
Hydrolysis Buffer 25 mLDevelopment Buffer 25 mLHydrolysis Enzyme Mix (Lyophilized) 1 vialDevelopment Enzyme Mix (Lyophilized) 1 vialDeveloper (Lyophilized) 1 vialGln Standard (Lyophilized) 1 vial
Detection method:
Colorimetric
Compatible Sample Types:
Biological fluids, Serum, Plasma, UrineMammalian tissues: brain samples, liver, kidney etc.


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Optional requirements on this protein +Expand
C-fusion   N-fusion  Non-tagged
His   GST  Fc  Others
<1.0 eu/μg   <0.1 eu/μg  <0.01 eu/μg  Not required
Monomer Isolation   Dimer Isolation   Not required
>80% by SDS-PAGE   >90% by SDS-PAGE  >95% by SDS-PAGE  Others
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45-1 Ramsey Road, Shirley, NY 11967, USA
USA: 1-631-559-9269  1-631-448-7888
Europe: 44-207-048-3343
FAX: 1-631-938-8127
EMAIL: info@creative-biomart.com

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