Address: 45-1 Ramsey Road, Shirley, NY 11967, USA    USA: 1-631-559-9269  1-631-448-7888   Fax: 1-631-938-8127  Europe: 44-207-048-3343   Email: info@creative-biomart.com
Home > Products > Assay Kits > Metabolism Kits > Glutathione Peroxidase Assay Kit II

Glutathione Peroxidase Assay Kit II

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Cat.No.:
Kit-0389
Product Overview:
Glutathione Peroxidase Assay Kit is used for measuring glutathione peroxidase (GPx) activity.
Description:
Glutathione Peroxidase (GPx, EC 1.11.1.9) family of enzymes play important roles in the protection of organisms from oxidative damage. GPx converts reduced glutathione (GSH) to oxidized glutathione (GSSG), to reduce lipid hydroperoxides to their corresponding alcohols or free hydrogen peroxide to water. Several isozymes have been found in different cellular locations and with different substrate specificity. Low levels of GPx have been correlated with free radical related disorders. In Glutathione Peroxidase Assay Kit, GPx reduces Cumene Hydroperoxide while oxidizing GSH to GSSG. The generated GSSG is reduced to GSH with consumption of NADPH by GR. The decrease of NADPH (easily measured at 340 nm) is proportionally to GPx activity. The assay can be used to measure all of the glutathione dependent peroxidases in plasma, erythrocyte lysates, tissue homogenates, and cell lysates with a detection sensitivity ~ 0.5 mU/mL of GPx in samples.
Target Species:
Mammals
Usage:
For research use only (RUO)
Storage:
Store the kit at -20°C, protect from light. Warm the assay buffer to room temperature before use. Briefly centrifuge vials before opening. Read the entire protocol before performing the assay.
Kit Components:
GPx Assay Buffer 25 mLNADPH (lyophilized) 1 vialGlutathione Reductase 1 vialGlutathione (GSH; lyophilized) 1 vialCumene Hydroperoxide 1 vialGPx Positive Control (lyophilized) 1 vial
Detection method:
Colorimetric
Compatible Sample Types:
Biological Fluid, Cell Culture Supernatant, Erythrocyte Lysate, Plasma, Serum, Tissue Culture Supernatant, Urine


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Optional requirements on this protein +Expand
C-fusion   N-fusion  Non-tagged
His   GST  Fc  Others
<1.0 eu/μg   <0.1 eu/μg  <0.01 eu/μg  Not required
Monomer Isolation   Dimer Isolation   Not required
>80% by SDS-PAGE   >90% by SDS-PAGE  >95% by SDS-PAGE  Others
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45-1 Ramsey Road, Shirley, NY 11967, USA
USA: 1-631-559-9269  1-631-448-7888
Europe: 44-207-048-3343
FAX: 1-631-938-8127
EMAIL: info@creative-biomart.com

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